This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
Actin-11
catalog :
AS10 702
quantity :
50 µl
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
IgG2b
reactivity :
Fusarium oxysporum, thale cress
application :
western blot, immunocytochemistry
citations: 3
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
| |
| Deng G, Yang Q, He W, Li C, Yang J, Zuo C, et al. Proteomic analysis of conidia germination in Fusarium oxysporum f. sp. cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana. Appl Microbiol Biotechnol. 2015;99:7189-207 pubmed publisher
|
product information
Product number :
AS10 702
Product name :
Actin-11
Immunogen :
full length ACT11 (Actin-11) from Arabidopsis thaliana, accession number NP_187818.1
Host :
Mouse
Clonality :
Monoclonal
Clone number :
IgG2b
Format :
Liquid, contains 50% glycerol
Quantity :
50 µl
Tested applications :
immunofluorescence (IF), Western blot (WB)
Related products :
AS13 2640 anti-ACT actin, rabbit antibody AS10 681 Anti-tubulin beta chain, rabbit antibodyAS10 680 Anti-tubulin alpha chain, rabbit antibodyAS16 3140 Actin-2, mouse monoclonal antibodyAS16 3141 Actin-8, mouse monoclonal antibodyAS16 3139 Actin-1, monoclonal antibodyPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 700 (IF), 1 : 2000 (WB)
Expected apparent MW :
41.6 45 kDa
Confirmed reactivity :
Arabidopsis thaliana, Fusarium oxysporum f.sp. cubense, Nepeta cataria, Nepeta rtanjensis, Nicotiana tabacum, Oryza sativa, Solanum tuberosum, Zea mays
Predicted reactivity :
Chlamydomonas reinhardtii, Glycine max, Picea abies, Pisum sativun, Ricinus communis
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
This antibody is not suitable as a loading control for tissues from various organs of cauliflower including seeds.
Selected references :
Zhao et al. (2017). MAP Kinase Cascades Regulate the Cold Response by Modulating ICE1 Protein Stability. Dev Cell. 2017 Dec 4;43(5):618-629.e5. doi: 10.1016/j.devcel.2017.09.024. Hashimoto et al. (2016). EXA1, a GYF domain protein, is responsible for loss-of-susceptibility to plantago asiatica mosaic virus in Arabidopsis thaliana. Plant J. 2016 Oct;88(1):120-131. doi: 10.1111/tpj.13265. Epub 2016 Sep 19. Deng et al. (2015). Proteomic analysis of conidia germination in Fusarium oxysporum f. sp. cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana. Appl Microbiol Biotechnol. 2015 Sep;99(17):7189-207. doi: 10.1007/s00253-015-6768-x. Epub 2015 Jul 1. Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64. Weiste and Dröge-Laser (2014). The Arabidopsis transcription factor bZIP11 activates auxin-mediated transcription by recruiting the histone acetylation machinery. Nat Commun. 2014 May 27;5:3883. doi: 10.1038/ncomms4883. Weits et al. (2014). Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway. Nat Commun. 2014 Mar 6;5:3425. doi: 10.1038/ncomms4425.
Pubmed ID's :
29056551, 27402258, 26129952, 24861440, 24599061
Background :
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Storage :
Store at -20°C. Shelf life of this product is one year from the date of shipment.
Additional information :
Antibody has been affinity purified.
TAIR number :
AT3G12110
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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