RA Rubisco activase | Agrisera AS10 700 product information

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product summary

company name :

Agrisera

product type :

antibody

product name :

RA Rubisco activase

catalog :

AS10 700

quantity :

50 µl

price :

302 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

citations: 7

Reference
Oikawa K, Imai T, Thagun C, Toyooka K, Yoshizumi T, Ishikawa K, et al. Mitochondrial movement during its association with chloroplasts in Arabidopsis thaliana. Commun Biol. 2021;4:292 pubmed publisher
Amiya R, Shapira M. ZnJ6 Is a Thylakoid Membrane DnaJ-Like Chaperone with Oxidizing Activity in Chlamydomonas reinhardtii. Int J Mol Sci. 2021;22: pubmed publisher
Suganami M, Suzuki Y, Kondo E, Nishida S, Konno S, Makino A. Effects of Overproduction of Rubisco Activase on Rubisco Content in Transgenic Rice Grown at Different N Levels. Int J Mol Sci. 2020;21: pubmed publisher
Salesse Smith C, Sharwood R, Busch F, Kromdijk J, Bardal V, Stern D. Overexpression of Rubisco subunits with RAF1 increases Rubisco content in maize. Nat Plants. 2018;4:802-810 pubmed publisher
Yoshida K, Hara A, Sugiura K, Fukaya Y, Hisabori T. Thioredoxin-like2/2-Cys peroxiredoxin redox cascade supports oxidative thiol modulation in chloroplasts. Proc Natl Acad Sci U S A. 2018;115:E8296-E8304 pubmed publisher
Tamburino R, Vitale M, Ruggiero A, Sassi M, Sannino L, Arena S, et al. Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017;17:40 pubmed publisher
Yin Y, Zhou Y, Zhou Y, Shi K, Zhou J, Yu Y, et al. Interplay between mitogen-activated protein kinase and nitric oxide in brassinosteroid-induced pesticide metabolism in Solanum lycopersicum. J Hazard Mater. 2016;316:221-31 pubmed publisher

product information

Product number :

AS10 700

Product name :

RA Rubisco activase

Quantity :

50 µl

List Price :

302 USD

Special price 2+ units (List Price) :

226

Special price 3+ units (List Price) :

205

Immunogen :

Purified, recombinant Rubisco activase from Gossypium hirsutum Q9AXG1

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Western blot (WB)

Recommended dilutions :

1 : 5000-1 : 10 000 (WB)

Expected apparent MW :

47 and 42 kDa (maize, tobacco, Chlamydomonas)

Confirmed reactivity :

Arabidopsis thaliana, Camelina sativa, Chlamydomonas reinhardtii, Caesalpinia pulcherrima, Hordeum spontaneum, Festuca pratensis, Glycine max, Gossypium hirsutum, Gossypium barbadense, Lolium perenne, Nannochloropsis oceanica, Nicotiana tabacum, Oryza sativa, Populus balsamifera, Rhoeo discolor, Solanum lycopersicum, Zea mays, Thellungiella salsuginea, red sulfur bacterium Thiodictyon sp. Cad16 (isolated from Lake Cadagno)

Predicted reactivity :

Glycine max, Gossypium mexicanum, Hordeum vulgare, Medicago sativa, Olea europea, Picea sitcHensis, Physcomitrium patens, Ricinus communis, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera

Not reactive in :

marine picocyanobacteria

Additional information :

There are two forms of activase (alpha and beta) in some species (for example Arabidopsis, camelina, spinach, rice) and only one form in other species (tobacco, maize, Chlamydomonas). Alpha is about 46-47 Kda, beta is about 42 kDa. Species that have only one form have the beta form.This product can be sold containing ProClin if requested

Related products :

Plant and algal protein extraction buffer Secondary antibodies

Selected references :

Amiya et al. (2021) Membrane DnaJ-Like Chaperone with Oxidizing Activity in Chlamydomonas reinhardtii. Int J Mol Sci. 2021 Jan 24;22(3):1136. doi: 10.3390/ijms22031136. PMID: 33498879; PMCID: PMC7865324.Oikawa et al. (2021) Mitochondrial movement during its association with chloroplasts in Arabidopsis thaliana. Commun Biol. 2021 Mar 5;4(1):292. doi: 10.1038/s42003-021-01833-8. PMID: 33674706.Wang et al. (2021) Insights Into the Gene Regulation in Jasmonate-Induced Whole-Plant Senescence of Tobacco Under Non-Starvation Condition. Plant Cell Physiol. 2021 Sep 15:pcab140. doi: 10.1093/pcp/pcab140. Epub ahead of print. PMID: 34523687.Trojak et al. (2021) Effects of partial replacement of red by green light in the growth spectrum on photomorphogenesis and photosynthesis in tomato plants. Photosynth Res. 2021 Sep 27. doi: 10.1007/s11120-021-00879-3. Epub ahead of print. PMID: 34580802.Yokochi et al.(2021) Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like proteins in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2021 Dec 21;118(51):e2114952118. doi: 10.1073/pnas.2114952118. PMID: 34907017; PMCID: PMC8713810.

Background :

RA - Ribulose bisphosphate carboxylase/oxygenase activase is an enzyme localized to chloroplasts which activates Rubisco by promoting ATP-dependent conformational changes. Alternative name: RuBisCo activase RCA.

Reconstitution :

For reconstitution add 50 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

This product can be sold containing ProClin if requested

UniProt number :

Q9AXG1

TAIR number :

AT2G39730

Commodity Code :

38220000

Our Category :

RUBISCO/Carbon metabolism

Research area :

Plant Biology, Photosynthesis