This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
AOX alternative oxidase
catalog :
AS10 699
quantity :
1,5 ml
price :
335.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
Podospora anserina, chickpea, thale cress
application :
western blot, immunoprecipitation
citations: 2
Published Application/Species/Sample/DilutionReference
  • western blot; Podospora anserina; 1:100; fig 2
Fischer F, Filippis C, Osiewacz H. RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model. Sci Rep. 2015;5:12697 pubmed publisher
  • western blot; chickpea; 1:5000; fig 1
Erdal S, Genisel M, Turk H, Dumlupinar R, Demir Y. Modulation of alternative oxidase to enhance tolerance against cold stress of chickpea by chemical treatments. J Plant Physiol. 2015;175:95-101 pubmed publisher
product information
Product number :
AS10 699
Product name :
AOX alternative oxidase
Price (EUR) :
335.00 USD
Immunogen :
alternative oxidase purified from Sauromatum guttatum (the voodoo lily)
Host :
mouse
Clonality :
monoclonal
Purity :
serum
Format :
liquid
Quantity :
1,5 ml
Tested applications :
western blot (WB), immunoprecipitation (IP), antibody column
Related products :
AS04 054 AOX1/2 plant alternative oxidase 1 and 2 (polyclonal antibody for higher plants)AS05 152 AOX1 alternative oxidase 1 (suitable for Chlamydomonas reinhardtii)Recommended secondary antibody (for western blot with ECL detection)
Recommended dilutions :
1 : 100 with standard ECL (WB, IP)
Expected apparent MW :
36-40 36-40 for Arabidopsis thaliana
Confirmed reactivity :
Arabidopsis thaliana, Glycine max (AOX1,2,3), Sauromatum guttatum, Solanum lycopersicum, Triticum aestivum
Predicted reactivity :
dicots including: Nicotiana tabacum, monocots including: Oryza sativa, diatoms, fungus Aspergillus sp., Neurospora crassa, yeast, protozoa: Trypanosoma sp.
Not reactive in :
no confirmed exceptions from predicted reactivity known in the moment
Storage :
store at -20°C; for long term storage at store at -80°C ; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Antibody is recognizing native and denatured AOX
Selected references :
Grabelnych et al. (2014). Mitochondrial Energy Dissipating Systems (Alternative Oxidase, Uncoupling Proteins, and External NADH Dehydrogenase) Are Involved in Development of Frost Resistance of Winter Wheat Seedlings. ISSN 0006 2979, Biochemistry (Moscow), 2014, Vol. 79, No. 6, pp. 506 519. © Pleiades Publishing, Ltd., 2014.
Background :
Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.
Special application note :
antibody is supplied in cell culture medium with 1mM sodium azide as preservativeProtocol for plant mitochondria isolation can be found here.
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specialized in polyclonal and monoclonal antibody production. We take pride in our high service level and our antibodies that really works. Nearly 30 years in business and close collaboration with research groups and pharmaceutical companies around the globe has taught us the tricks of the trade.