AGO9 Argonaute 9 | Agrisera AS10 673 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

AGO9 Argonaute 9

catalog :

AS10 673

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, immunoprecipitation

more info or order :

Agrisera product webpage

citations: 1

Reference
Su Z, Zhao L, Zhao Y, Li S, Won S, Cai H, et al. The THO Complex Non-Cell-Autonomously Represses Female Germline Specification through the TAS3-ARF3 Module. Curr Biol. 2017;27:1597-1609.e2 pubmed publisher

product information

Product number :

AS10 673

Product name :

AGO9 Argonaute 9

Immunogen :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO9 protein sequence UniProt: Q84YI4, TAIR:At5g21150.

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Immunogen affinity purified serum in PBS pH 7.4.

Format :

Lyophilized

Quantity :

100 µg

Tested applications :

Immunolocalization, whole-mount (IL), Immunoprecipitation (IP), Western blot (WB)

Related products :

AS09 527 Anti-AGO1 argonaute 1, rabbit antibodyAS09 527-ALP Anti-AGO1 argonaute 1 (40 µg, ALP-conjugated) AS09 527B Anti-AGO1 argonaute 1 (40 µg, Biotin conjugated) AS09 527-HRP Anti-AGO1 argonaute 1 (40 µg, HRP-conjugated) AS14 2776 Anti-AGO1 argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P AGO1 argonaute 1 Blocking peptideAS13 3682 Anti-AGO2 argonaute 2, rabbit antibodyAS09 617 Anti-AGO4 argonaute 4, rabbit antibodyAS10 671 Anti-AGO5 argonaute 5, rabbit antibodyAS10 672 Anti-AGO6 argonaute 6, rabbit antibodyAS15 3071 Anti-AGO10 argonaute 10, rabbit antibodycollection of antibodies to micro RNAPlant protein extraction buffer

Recommended dilutions :

5 µg of antibody per 1 gram of a fresh tissue (IP), 1 : 10 000 (WB)

Expected apparent MW :

101 kDa

Confirmed reactivity :

Arabidopsis thaliana

Predicted reactivity :

Arabidopsis thaliana

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known

Additional information :

AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

Selected references :

Hou et al. (2021) High-throughput single-cell transcriptomics reveals the female germline differentiation trajectory in Arabidopsis thaliana. Commun Biol. 2021 Oct 1;4(1):1149. doi: 10.1038/s42003-021-02676-z. PMID: 34599277; PMCID: PMC8486858. (immunolocalization)Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956Su et al. (2017). The THO Complex Non-Cell-Autonomously Represses Female Germline Specification through the TAS3-ARF3 Module. Curr Biol. 2017 Jun 5;27(11):1597-1609.e2. doi: 10.1016/j.cub.2017.05.021.Havecker et al. (2010) The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell 22(2): 321-34.

Background :

AGO9 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC).

Reconstitution :

For reconstitution add 100 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

UniProt number :

Q84YI4

TAIR number :

AT5G21150

Research area :

Arabidopsis thaliana

more info or order :

Agrisera product webpage