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company name :
Agrisera
product type :
antibody
product name :
AGO6 Argonaute 6
catalog :
AS10 672
quantity :
50 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS10 672
Product name :
AGO6 Argonaute 6
Immunogen :
KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Western blot (WB)
Related products :
AS09 527 Anti-AGO1 argonaute 1, rabbit antibodyAS09 527-ALP Anti-AGO1 argonaute 1 (40 µg, ALP-conjugated) AS09 527B Anti-AGO1 argonaute 1 (40 µg, Biotin conjugated) AS09 527-HRP Anti-AGO1 argonaute 1 (40 µg, HRP-conjugated) AS14 2776 Anti-AGO1 argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P AGO1 argonaute 1 Blocking peptideAS13 3682 Anti-AGO2 argonaute 2, rabbit antibodyAS09 617 Anti-AGO4 argonaute 4, rabbit antibodyAS10 671 Anti-AGO5 argonaute 5, rabbit antibodyAS10 673 Anti-AGO9 argonaute 9, rabbit antibodyAS15 3071 Anti-AGO10 argonaute 10, rabbit antibodycollection of antibodies to micro RNAPlant protein extraction buffer
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
116,4 99 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Arabidopsis thaliana
Not reactive in :
Zea mays
Additional information :
AGO6 expression is cell specific, Seedlings can be used as a negative control
Selected references :
Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956 Havecker el al. (2010). The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell. 2010 Feb;22(2):321-34. doi: 10.1105/tpc.109.072199.
Background :
AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
There can be a reaction with NEB broad range prestained protein ladder depending upon secondary antibody used.AGO6 accumulates to lower levels in the nrpd1a mutant (Havecker at el. 2010). In addition, a non-specific band migrates to a similar location and thus protein was run on a 6% denaturing gel to obtain good separation.If other secondary antibody is used than in the figure below, cross-reacting pattern can be different.
UniProt number :
O48771
TAIR number :
At2g32940
Research area :
Arabidopsis thaliana
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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