product summary
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company name :
Agrisera
product type :
antibody
product name :
AGO5 Argonaute 5
catalog :
AS10 671
quantity :
50 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
product information
Product number :
AS10 671
Product name :
AGO5 Argonaute 5
Immunogen :
peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Immunofluorescebce (IF), Immunoprecipitation (IP), Western blot (WB)
Related products :
AS09 527 Anti-AGO1 argonaute 1, rabbit antibodyAS09 527-ALP Anti-AGO1 argonaute 1 (40 µg, ALP-conjugated) AS09 527B Anti-AGO1 argonaute 1 (40 µg, Biotin conjugated) AS09 527-HRP Anti-AGO1 argonaute 1 (40 µg, HRP-conjugated) AS14 2776 Anti-AGO1 argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P AGO1 argonaute 1 Blocking peptideAS13 3682 Anti-AGO2 argonaute 2, rabbit antibodyAS09 617 Anti-AGO4 argonaute 4, rabbit antibodyAS10 672 Anti-AGO6 argonaute 6, rabbit antibodyAS10 673 Anti-AGO9 argonaute 9, rabbit antibodyAS15 3071 Anti-AGO10 argonaute 10, rabbit antibodycollection of antibodies to micro RNAPlant protein extraction buffer
Recommended dilutions :
1 : 100 (IF), 5 ug per gram floral tissue (IP), 1 : 1500 (WB)
Expected apparent MW :
111 111 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Arabidopsis thaliana
Not reactive in :
Hordeum vulgare, Solanum lycopersicum, Zea mays
Additional information :
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure
Selected references :
Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.
Background :
AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). Probably involved in antiviral RNA silencing.
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
UniProt number :
Q9SJK3
TAIR number :
AT2G27880
Research area :
Arabidopsis thaliana
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.