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company name :
Agrisera
product type :
antibody
product name :
Goat anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to bovine/Human/goat/mouse/rat IgG
catalog :
AS10 667
quantity :
1 mg
price :
206 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
application :
western blot, ELISA, immunohistochemistry
more info or order :
Agrisera product webpage
product information
Product number :
AS10 667
Product name :
Goat anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to bovine/Human/goat/mouse/rat IgG
Quantity :
1 mg
List Price :
206 USD
Immunogen :
Purified Rabbit IgG, whole molecule (H&L chains)
Host :
Goat
Clonality :
Polyclonal
Purity :
Immunogen affinity purified goat IgG.
Format :
Lyophilized
Tested applications :
Immunohistochemistry (IHC), ELISA (ELISA), Western blot (WB)
Recommended dilutions :
1 : 200- 1 : 5 000 (ELISA), 1 : 500- 1 : 5 000 (IHC), 1 : 200- 1 : 5 000 (WB)
Confirmed reactivity :
Rabbit IgG heavy and light chains (H&L)
Predicted reactivity :
Rabbit IgG Heavy and Light chains (H&L)
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis,
Background :
Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Reconstitution :
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage :
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Additional information :
No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis.No reactivity to non-immunoglobulin rabbit serum proteins.Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.
Commodity Code :
38220000
Our Category :
HRP (horse radish peroxidase)
Research area :
Immunoassays
more info or order :
Agrisera product webpage
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
https://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.