product summary
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company name :
Agrisera
product type :
antibody
product name :
STN8 Serine/threonine-protein kinase STN8 (chloroplastic)
catalog :
AS10 1601
quantity :
50 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS10 1601
Product name :
STN8 Serine/threonine-protein kinase STN8 (chloroplastic)
Immunogen :
KLH-conugated synthetic peptide (amino acids 425 - 438) specific for Arabidopsis thaliana STN8 serine/threonine proteinkinase, UniProt: Q9LZV4 TAIR:At5g01920
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Western blot (WB)
Related products :
AS15 3080 Anti-STN7 serine/threonine-protein kinase STN7, chloroplastic for Chlamydomonas reinhardtii, rabbit antibodiesSecondary antibodies
Recommended dilutions :
1 : 2000 (WB)
Expected apparent MW :
54,9 kDa or 46 kDa on 6 M urea gel
Confirmed reactivity :
Arabidopsis thaliana, Triticum aestivum
Predicted reactivity :
Oryza sativa Species of your interest not listed? Contact us
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known
Additional information :
For best results with this antibody sample buffer needs to contain 6 M urea (138mM TrisHCl pH 6.8, 6M urea, 22.2% Glycerol, 4.3% SDS).STN8 is a nuclear encoded protein which is localized in chloroplast, hence posses a chloroplastic target peptides (cTP) at the beginning of the amino acid sequence which is cut off. Accroding to TargetP (program that predicts the length of the cTP:s) the length of cTP for Stn8 is 49 amino acids. STN8 portein mobility can be also affected by urea present in the gel.Due to a high background signal with LHCII it is adviced to cut off a membrane below 30 kDa marker.
Selected references :
Li et al. (2015). Effect of hydrogen sulfide on D1 protein in wheat under drought stress. Acta Physiologiae Plantarum November 2015, 37:225.Flood et al. (2014). Natural variation in phosphorylation of photosystem II proteins in Arabidopsis thaliana: is it caused by genetic variation in the STN kinases? Philos Trans R Soc Lond B Biol Sci. 2014 Mar 3;369(1640):20130499. doi: 10.1098/rstb.2013.0499. Print 2014.Yin et al. (2012). Photosystem II Function and Dynamics in Three Widely Used Arabidopsis thaliana Accessions. PLOS ONE, open access.
Background :
Serine/threonine protein kinase (EC=2.7.11.1), known as STN8 in Arabidopsis, and its Stl1 homologue in Chlamydomonas, are STN7/Stt7-like proteins that are not required for state transitions. The Arabidopsis mutants deficient in stn8 gene demonstrated highly reduced levels of the phosphorylated proteins of the photosystem II (Bonardi et al., 2005 Varionen et al., 2005). Alternative name: Protein STATE TRANSITION 8.
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
An extract from STN8 mutant needs to be used in pararel to determine specific band of STN8 protein on a western blot
UniProt number :
Q9LZV4
TAIR number :
AT5G01920
Research area :
Arabidopsis thaliana
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.