product summary
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company name :
Agrisera
product type :
antibody
product name :
Ricin (RTA subunit), clone MMA
catalog :
AS09 648
quantity :
200 µg
price :
407 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MMA
application :
ELISA, immunohistochemistry
more info or order :
product information
Product number :
AS09 648
Product name :
Ricin (RTA subunit), clone MMA
Quantity :
200 µg
List Price :
407 USD
Immunogen :
Ricin, RTA subunit
Host :
Mouse
Clonality :
Monoclonal
Isotype :
IgG1
Purity :
Purified IgG in PBS pH 7.4 with mannitol.
Format :
Lyophilized
Tested applications :
ELISA (ELISA), Immunohistochemistry (IHC)
Recommended dilutions :
The optimal working dilution should be determined by the investigator, for specific application
Expected apparent MW :
29.8 kDa
Confirmed reactivity :
Ricinus communis
Predicted reactivity :
Ricinus communis
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Detected epitope is located on the surface of RTA subunit not accssible in intact ricin molecule, This antibody does not cross-react to whole ricin molecule
Related products :
Secondary antibodies
Background :
Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell.
Reconstitution :
For reconstitution add 200 µl of sterile water
Storage :
Store at -70°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4°C sodium azide can be added
Additional information :
Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant and lyophilized from solution containing: mannitol 1%, dextran 1%, NaCl 100mM, sodium phosphate 10 mM, pH 7.5. If required, mannitol can be removed either by dialysis or by chromatography using mini columns Sephadex G50 fine. The concentration can be measured by absorbance at 280 nm (1.35 for 1 mg/ml) or by Bradford methodRicin detection limit is 5 ng/ml, signal-to-noise ratio is 15-50 up to 100 ng/mlEpitope recognized by this antibody is located on the surface of RTA subunit and not accessible in intact ricin molecule. Suggested protocol can be found here.
Commodity Code :
38220000
Our Category :
Plant toxins
Research area :
Immunology, Allergy
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.