product summary
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company name :
Agrisera
product type :
antibody
product name :
AGO4 Argonaute 4
catalog :
AS09 617
quantity :
50 µg
price :
302 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
citations: 3
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product information
Product number :
AS09 617
Product name :
AGO4 Argonaute 4
Quantity :
50 µg
List Price :
302 USD
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
Immunogen :
KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5 , TAIR: At2g27040
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Tested applications :
CoImmunoprecipitation (IP) (coIP), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western blot (WB)
Recommended dilutions :
1 : 100 (ICC), 5 µg of antibody per 1 gram of a fresh tissue (IP),1 : 2000-1 : 5000 (WB)
Expected apparent MW :
103 kDa
Confirmed reactivity :
Arabidopsis thaliana, Hyacinthus orientalis
Predicted reactivity :
Arabidopsis thaliana
Not reactive in :
Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Solanum lycopersicum, Vigna angularis, Zea mays
Additional information :
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Binds endogenous siRNAs, preference for 24nt siRNAs with 5' A.Note that the AGO4 antibody reacts with the NEB prestained protein marker.
Related products :
Secondary antibodies
Selected references :
Clavel et al. (2021) Atypical molecular features of RNA silencing against the phloem-restricted polerovirus TuYV. Nucleic Acids Res. 2021 Nov 8;49(19):11274-11293. doi: 10.1093/nar/gkab802. PMID: 34614168; PMCID: PMC8565345.Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Niedojad?o et al. (2020). Dynamic distribution of ARGONAUTE1 (AGO1) and ARGONAUTE4 (AGO4) in Hyacinthus orientalis L. pollen grains and pollen tubes growing in vitro. Protoplasma. 2020 Jan 8. doi: 10.1007/s00709-019-01463-2.Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956Yang et al. (2017). The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation loci. Genome Biol. 2017 May 31;18(1):103. doi: 10.1186/s13059-017-1226-y.
Background :
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tube.
UniProt number :
Q9ZVD5
TAIR number :
AT2G27040
Commodity Code :
38220000
Our Category :
plant RNA
Research area :
RNAi/siRNA/miRNA
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.