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company name :
Agrisera
product type :
antibody
product name :
V-ATPase Epsilon subunit of tonoplast H+ATPase (goat antibody)
catalog :
AS09 577
quantity :
300 µl
price :
394 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
Agrisera product webpage
product information
Product number :
AS09 577
Product name :
V-ATPase Epsilon subunit of tonoplast H+ATPase (goat antibody)
Quantity :
300 µl
List Price :
394 USD
Immunogen :
KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
Host :
Goat
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Tested applications :
Western blot (WB)
Recommended dilutions :
1 : 1000-1 : 3000 (WB)
Expected apparent MW :
26 31 kDa (Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Avena strigosa, Nicotiana tabacum, Solanum lycopersicum
Predicted reactivity :
Algae, Chlamydomonas reinhardtii, Hordeum vulgare, Malus domestica, Mesembryanthemum sp., Oryza sativa, Petunia sp.,Phaseolus sp. , Physcomitrium patens, Pteris vittata (fern), Ricinus communis, Thellungiella sp., Zea mays, Vitis vinifera Bull frog, Chicken, Bovine, Drosophila melanogaster, Human, Mouse, Rat
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.
Related products :
Plant protein extraction bufferSecondary antibodies
Selected references :
McLoughlin et al. (2012). TheSnf1-relatedproteinkinasesSnRK2.4 andSnRK2.10 areinvolved inmaintenance ofrootsystemarchitecture duringsaltstress. Plant J. June 2012.
Background :
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
Reconstitution :
For reconstitution add 300 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
UniProt number :
Q39258
TAIR number :
AT4G11150
Commodity Code :
38220000
Our Category :
Vacuolar membrane
Research area :
Plant Biology
more info or order :
Agrisera product webpage
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
https://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.