antibody

AGO1 Argonaute 1

AS09 527

50 µg

polyclonal

rabbit

nonconjugated

western blot, chromatin immunoprecipitation

AGO1 Argonaute 1

N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410

Rabbit

Polyclonal

Affinity purified serum in PBS pH 7.4.

Lyophilized

50 µg

Chromatin ImmunoPrecipiation (ChIP), Immunolocalization (IL), small-RNA-IP-Seq, Western blot (WB)

AS09 527-ALP Anti-AGO1 Argonaute 1 (40 µg, ALP-conjugated) AS09 527B Anti-AGO1 Argonaute 1 (40 µg, Biotin conjugated) AS09 527-HRP Anti-AGO1 Argonaute 1 (40 µg, HRP-conjugated) AS14 2776 Anti-AGO1 Argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P AGO1 Argonaute 1 Blocking peptideAS13 3682 Anti-AGO2 Aargonaute 2, rabbit antibodyAS09 617 Anti-AGO4 Argonaute 4, rabbit antibodyAS10 671 Anti-AGO5 Argonaute 5, rabbit antibodyAS10 672 Anti-AGO6 Argonaute 6, rabbit antibodyAS10 673 Anti-AGO9 Argonaute 9, rabbit antibodyAS15 3071 Anti-AGO10 Argonaute 10, rabbit antibodyCollection of antibodies to micro RNAPlant protein extraction bufferSecondary antibodies

2 µg (ChIP), 1: 200 (IL), small-RNA-IP-Seq, 1 : 5000-1 : 10 000 (WB)

116.4 130 kDa

Arabidopsis thaliana, Nicotiana benthamiana

Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera

Chlamydomonas reinhardtii, Triticum aestivum

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Buffer for extraction of AGO proteins: Paudel et al. 2018. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.TCA acetone precipitation method

Chen et al. (2018). Structural and biochemical insights into small RNA 3' end trimming by Arabidopsis SDN1. Nat Commun. 2018 Sep 4;9(1):3585. doi: 10.1038/s41467-018-05942-7. (Immunoprecipitation)Bologna et al. (2018). Nucleo-cytosolic Shuttling of ARGONAUTE1 Prompts a Revised Model of the Plant MicroRNA Pathway. Mol Cell. 2018 Feb 15;69(4):709-719.e5. doi: 10.1016/j.molcel.2018.01.007. Zhang et al. (2017). RISC-interacting clearing 3'- 5' exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis thaliana. Elife. 2017 May 2;6. pii: e24466. doi: 10.7554/eLife.24466. Schalk et al. (2017). Small RNA-mediated repair of UV-induced DNA lesions by the DNA DAMAGE-BINDING PROTEIN 2 and ARGONAUTE 1. Proc Natl Acad Sci U S A. 2017 Mar 21. pii: 201618834. doi: 10.1073/pnas.1618834114.Dolata et al. (2016). Salt Stress Reveals a New Role for ARGONAUTE1 in miRNA Biogenesis at the Transcriptional and Posttranscriptional Levels. Plant Physiol. 2016 Sep;172(1):297-312. doi: 10.1104/pp.16.00830. (ChIP and Immunolocalization)Pumplin et al. (2016). DNA Methylation Influences the Expression of DICER-LIKE4 Isoforms, Which Encode Proteins of Alternative Localization and Function. The Plant Cell November 14, 2016 tpc.00554.2016 . Advance Publication November 14, 2016.Minoia et al. (2014). Specific Argonautes Selectively Bind Small RNAs Derived from Potato Spindle Tuber Viroid and Attenuate Viroid Accumulation In Vivo. J Virol. 2014 Oct 15;88(20):11933-45. doi: 10.1128/JVI.01404-14. Epub 2014 Aug 6.Haveckeret al. (2010). The Arabidopsis RNA-directed DNA methylation argonautes functionally diverge based on their expression and interaction with target loci. Plant Cell. 2010 Feb;22(2):321-34. doi: 10.1105/tpc.109.072199. (small-RNA-IP-Seq)

30181559, 29398448, 28463111, 28325872, 27385819, 25100851, 20173091

AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.To detect AGO1 in Nicotiana benthamiana, please inquire.

O04379

AT1G48410