product summary
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company name :
Agrisera
product type :
antibody
product name :
AGO1 Argonaute 1, DyLight® 594 conjugated (40 µg)
catalog :
AS09 527-DL594
quantity :
40 µg
price :
448 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
immunocytochemistry
more info or order :
Agrisera product webpage
product information
Product number :
AS09 527-DL594
Product name :
AGO1 Argonaute 1, DyLight® 594 conjugated (40 µg)
Quantity :
40 µg
List Price :
448 USD
Immunogen :
KLH-conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum
Format :
Liquid in PBS pH 7.4.
Tested applications :
Immunofluorescence (IF)
Expected apparent MW :
116,4 130 kDa
Confirmed reactivity :
Arabidopsis thaliana, Nicotiana benthamiana
Predicted reactivity :
Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Not reactive in :
Chlamydomonas reinhardtii
Additional information :
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure,The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies, The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot,
Background :
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi),This antibody is directly conjugated to Horseradish Peroxidase (HRP),
Storage :
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years,
Additional information :
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U,TCA acetone total protein precipitation method
UniProt number :
O04379
TAIR number :
AT1G48410
Commodity Code :
38220000
Our Category :
plant RNA
Research area :
RNAi/siRNA/miRNA
more info or order :
Agrisera product webpage
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
https://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.