MnSOD Manganese superoxide dismutase | Agrisera AS09 524 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

MnSOD Manganese superoxide dismutase

catalog :

AS09 524

quantity :

50 µl

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

Pelargonium zonale, thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; scFv; 1:5000; fig 2	Vuleta A, ManitaÅ¡eviÄ‡ JovanoviÄ‡ S, TuciÄ‡ B. Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016;100:166-173 pubmed publisher
western blot; Pelargonium zonale; 1:2000; fig 2	VidoviÄ‡ M, Morina F, MiliÄ‡ KomiÄ‡ S, Vuleta A, Zechmann B, ProkiÄ‡ L, et al. Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants. Plant Biol (Stuttg). 2016;18:669-80 pubmed publisher

product information

Product number :

AS09 524

Product name :

MnSOD Manganese superoxide dismutase

Immunogen :

KLH-conjugated synthetic peptide derived from available MnSOD sequences in di and monocotyl plants including Arabidopsis thaliana O81235, At3g10920

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

50 µl

Tested applications :

Western blot (WB)

Related products :

AS06 125 anti-FeSOD chloroplastic Fe-dependent superoxide dismutaseAS06 170 anti-CSD2 chloroplastic Cu/Zn superoxide dismutaseAS07 219 anti-CCS chloroplastic copper chaperone for SODPlant protein extraction bufferSecondary antibodies

Recommended dilutions :

1 : 2000-1 : 5000 (WB)

Expected apparent MW :

25 25 kDa

Confirmed reactivity :

Arabidopsis thaliana, Armeria maritima, Brassica napus, Brassica oleracea, Iris pumila, Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Pisum sativum, Salicornia sp. , Solanum tuberosum

Predicted reactivity :

Gossipium mexicanum, Hordeum vulgare, Musa acuminata, Picea sitcHensis, Populus balsamifera sub. trichocarpa, Raphanus sativus, Triticum aestivum, Vitis vinifera, Zea mays

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Selected references :

BalÃ¡Å¾ovÃ¡ et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013 Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe DBalÃ¡Å¾ovÃ¡ rought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1). Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19DmitroviÄ‡ et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64.Dimkovikj and Van Hoewyk (2014). Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response. BMC Plant Biol. 2014 Sep 30;14:259. doi: 10.1186/s12870-014-0259-6.Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29.MomÄiloviÄ‡ et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.

Pubmed ID's :

29957573, 26520835, 27124767, 26841194, 25267309, 25070267

Background :

Plant MnSOD catalyzes the dismutation of superoxide to molecular oxygen and water. One subunit of the homotetramer complex binds 1 Mn cofactor. Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD. Synonymes: MSD1, SODA, SOD3

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

Freshly prepared reducing agent like DTT needs to be used in a sample buffer. Otherwise MnSOD will migrate at 50 kDa.

UniProt number :

O81235

TAIR number :

AT3G10920

more info or order :

Agrisera product webpage