Lhcb1 LHCII type I chlorophyll a/b-binding protein (Arabidopsis specific)

product summary

company name :

Agrisera

product type :

antibody

product name :

Lhcb1 LHCII type I chlorophyll a/b-binding protein (Arabidopsis specific)

catalog :

AS09 522

quantity :

100 µg

price :

369 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 5

Reference
Pralon T, Shanmugabalaji V, Longoni P, Glauser G, Ksas B, Collombat J, et al. Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019;2:220 pubmed publisher
Lal N, Nagalakshmi U, Hurlburt N, Flores R, Bak A, Sone P, et al. The Receptor-like Cytoplasmic Kinase BIK1 Localizes to the Nucleus and Regulates Defense Hormone Expression during Plant Innate Immunity. Cell Host Microbe. 2018;23:485-497.e5 pubmed publisher
Fristedt R, Trotta A, Suorsa M, Nilsson A, Croce R, Aro E, et al. PSB33 sustains photosystem II D1 protein under fluctuating light conditions. J Exp Bot. 2017;68:4281-4293 pubmed publisher
Hartings S, Paradies S, Karnuth B, Eisfeld S, Mehsing J, Wolff C, et al. The DnaJ-Like Zinc-Finger Protein HCF222 Is Required for Thylakoid Membrane Biogenesis in Plants. Plant Physiol. 2017;174:1807-1824 pubmed publisher
Tamburino R, Vitale M, Ruggiero A, Sassi M, Sannino L, Arena S, et al. Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017;17:40 pubmed publisher

product information

Product number :

AS09 522

Product name :

Lhcb1 LHCII type I chlorophyll a/b-binding protein (Arabidopsis specific)

Quantity :

100 µg

List Price :

369 USD

Immunogen :

BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430 (Lhcb1.4), and At2g34420 (Lhcb1.5)

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Immunogen affinity purified serum in PBS pH 7.4.

Format :

Lyophilized

Tested applications :

Western blot (WB)

Recommended dilutions :

1 : 2500-1 : 5000 (WB)

Expected apparent MW :

28 25 kDa (Arabidopsis thaliana)

Confirmed reactivity :

Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Pinus strobus L.

Predicted reactivity :

Algae, Dicots, Mosses Species of your interest not listed? Contact us

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

This Lhcb1 antibody is directed specifically against the Arabidopsis Lhcb1 gene products, for those that would prefer higher specific activity over broader specificity offered by Agrisera older Lhcb1 antibody, AS01 004Protein is processed into mature form (Jansson 1999).

Related products :

Plant protein extraction bufferSecondary antibodies

Selected references :

Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254. Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4. Lal et al. (2018). The Receptor-like Cytoplasmic Kinase BIK1 Localizes to the Nucleus and Regulates Defense Hormone Expression during Plant Innate Immunity. Cell Host Microbe. 2018 Apr 11;23(4):485-497.e5. doi: 10.1016/j.chom.2018.03.010.Tamburino et al. (2017). Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017 Feb 10;17(1):40. doi: 10.1186/s12870-017-0971-0.Fristedt et al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218.Hartings et al. (2017). The DnaJ-Like Zinc-Finger Protein HCF222 Is Required for Thylakoid Membrane Biogenesis in Plants. Plant Physiol. 2017 Jul;174(3):1807-1824. doi: 10.1104/pp.17.00401.Correa-Galvis et al. (2016). PsbS interactions involved in the activation of energy dissipation in Arabidopsis. Nature Plants 2, Article number: 15225 (2016) doi:10.1038/nplants.2015.225Longoni et al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015.Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.

Background :

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.

Reconstitution :

For reconstitution add 100 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1-3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476

UniProt number :

Q8VZ87 , P04777 , P04778 , Q9C5R6 , Q39141

TAIR number :

AT1G29910, AT1G29920, AT1G29930, AT2G34420, AT2G34430

Commodity Code :

38220000

Our Category :

LHC

Research area :

Plant Biology, Photosynthesis

more info or order :

Agrisera product webpage