product summary
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company name :
Agrisera
product type :
antibody
product name :
AKT1 Potassium channel AKT1
catalog :
AS09 516
quantity :
200 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot
more info or order :
product information
Product number :
AS09 516
Product name :
AKT1 Potassium channel AKT1
Immunogen :
KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum
Format :
Lyophilized
Quantity :
200 µg
Tested applications :
Western blot (WB)
Related products :
AS09 515 anti-KC1 potassium channel KAT3collection of antibodies to membrane transport systemPlant protein extraction bufferSecondary antibodies
Recommended dilutions :
1 : 50 with 125I (WB)
Expected apparent MW :
96.9 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Arabidopsis thaliana
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
Selected references :
Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. doi: 10.3389/fpls.2015.00047. Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.
Pubmed ID's :
19794113
Background :
AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
Reconstitution :
For reconstitution add 100 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1.
UniProt number :
Q38998
TAIR number :
AT2G26650
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.