product summary
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company name :
Agrisera
product type :
antibody
product name :
KC1 potassium channel KAT3
catalog :
AS09 515
quantity :
200 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS09 515
Product name :
KC1 potassium channel KAT3
Immunogen :
KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum
Format :
Lyophilized
Quantity :
200 µg
Tested applications :
Western blot (WB)
Related products :
AS09 516 anti-AKT1 potassium channel AKT1collection of antibodies to membrane transport systemPlant protein extraction bufferSecondary antibodies
Recommended dilutions :
1 : 50 with 125I (WB)
Expected apparent MW :
75.5 75 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Arabidopsis thaliana
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
Selected references :
Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.
Pubmed ID's :
19794113
Background :
KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
Reconstitution :
For reconstitution add 100 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
For detection images please, refer to the publication belowAntibody detects native and recombinant KC1.
UniProt number :
P92960
TAIR number :
AT4G32650
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.