PIP2;3 Aquaporin, plasma membrane intrinistic protein 2-3 | Agrisera AS09 506 product information

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product summary

company name :

Agrisera

product type :

antibody

product name :

PIP2;3 Aquaporin, plasma membrane intrinistic protein 2-3

catalog :

AS09 506

quantity :

100 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot, ELISA

product information

Product number :

AS09 506

Product name :

PIP2;3 Aquaporin, plasma membrane intrinistic protein 2-3

Immunogen :

KLH-conjugated peptide derived from Oryza sativa PIP2;3, UniProt: Q7XUA6

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

100 µl

Tested applications :

Chromogenic Immunohistochemistry (CIH), ELISA (ELISA), Immunogold (IG), Western blot (WB)

Related products :

collection of antibodies to plasma membrane proteins AS09 505 Anti-PIP1s aquaporin, rabbit antibodiesAS09 507 Anti-PIP2;1 aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodiesAS09 508 Anti-PIP2;5 aquaporin, plasma membrane intrinistic protein 2-5, rabbit antibodiesPlant protein extraction buffer

Recommended dilutions :

1: 50 (CIH), 1 : 8000 (ELISA), 1 : 100 (IG), 1 : 2000 (WB)

Expected apparent MW :

31,9 26-29 kDa

Confirmed reactivity :

Oryza sativa, Zea mays

Predicted reactivity :

Species of your interest not listed? Contact us

Not reactive in :

Arabidopsis thaliana, Hordeum vulgare, Solanum tuberosum

Additional information :

Antibody works very well on samples from roots. Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Selected references :

Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.

Background :

PIP2;3 is an aquaporin which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: plasma membrane intrinsic protein 2-3, OsPIP2;3

Reconstitution :

For reconstitution add 100 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient. This antibody will recognize PIP2;3 in root tissue only. No signal is obtained for leaf tissue. For immunogold technique, secondary antibodies conjugated with coloidal gold, 10 nm particle size were used.

UniProt number :

Q6Z2T3

Research area :

Oryza sativa