product summary
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company name :
Agrisera
product type :
antibody
product name :
Cat Catalase (peroxisomal marker)
catalog :
AS09 501
quantity :
50 µl
price :
302 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, immunoprecipitation
more info or order :
citations: 19
Published Application/Species/Sample/DilutionReference
  • western blot; thale cress; loading ...; fig 4d
Bi C, Ma Y, Wu Z, Yu Y, Liang S, Lu K, et al. Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination. Plant Mol Biol. 2017;94:197-213 pubmed publisher
Kalemba E, Alipour S, Wojciechowska N. NAD(P)H Drives the Ascorbate-Glutathione Cycle and Abundance of Catalase in Developing Beech Seeds Differently in Embryonic Axes and Cotyledons. Antioxidants (Basel). 2021;10: pubmed publisher
Li X, Chai Y, Yang H, Tian Z, Li C, Xu R, et al. Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021;8:31 pubmed publisher
Tokarz K, Wesołowski W, Tokarz B, Makowski W, Wysocka A, Jędrzejczyk R, et al. Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021;22: pubmed publisher
Tokarz K, Makowski W, Tokarz B, Hanula M, Sitek E, Muszyńska E, et al. Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity?-Studies of Photosynthetic Apparatus Efficiency. Int J Mol Sci. 2020;21: pubmed publisher
Boussardon C, Przybyla Toscano J, Carrie C, Keech O. Tissue-specific isolation of Arabidopsis/plant mitochondria - IMTACT (isolation of mitochondria tagged in specific cell types). Plant J. 2020;103:459-473 pubmed publisher
Mares J, Gramacho K, Santana J, Oliveira de Souza A, Alvim F, Pirovani C. Hydrosoluble phylloplane components of Theobroma cacao modulate the metabolism of Moniliophthora perniciosa spores during germination. Fungal Biol. 2020;124:73-81 pubmed publisher
Calero Muñoz N, Expósito Rodríguez M, Collado Arenal A, Rodríguez Serrano M, Laureano Marín A, Santamaría M, et al. Cadmium induces reactive oxygen species-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019;42:2696-2714 pubmed publisher
Szymańska K, Polkowska Kowalczyk L, Lichocka M, Maszkowska J, Dobrowolska G. SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019;20: pubmed publisher
Pan J, Li Z, Wang Q, Garrell A, Liu M, Guan Y, et al. Comparative proteomic investigation of drought responses in foxtail millet. BMC Plant Biol. 2018;18:315 pubmed publisher
Bastow E, García de la Torre V, Maclean A, Green R, Merlot S, Thomine S, et al. Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018;177:1267-1276 pubmed publisher
Su T, Wang P, Li H, Zhao Y, Lu Y, Dai P, et al. The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome-derived signaling in plant development. J Integr Plant Biol. 2018;60:591-607 pubmed publisher
Kang S, Shin K, Kim J, Chung T. Autophagy-related (ATG) 11, ATG9 and the phosphatidylinositol 3-kinase control ATG2-mediated formation of autophagosomes in Arabidopsis. Plant Cell Rep. 2018;37:653-664 pubmed publisher
Mares J, Gramacho K, Santos E, da Silva Santiago A, Santana J, de Sousa A, et al. Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches' broom disease in cacao. BMC Microbiol. 2017;17:176 pubmed publisher
Kneeshaw S, Keyani R, Delorme Hinoux V, Imrie L, Loake G, Le Bihan T, et al. Nucleoredoxin guards against oxidative stress by protecting antioxidant enzymes. Proc Natl Acad Sci U S A. 2017;: pubmed publisher
Zhang Y, Wang G, Song L, Mu P, Wang S, Liang W, et al. Global analysis of protein lysine succinylation profiles in common wheat. BMC Genomics. 2017;18:309 pubmed publisher
Dauphinee A, Fletcher J, Denbigh G, Lacroix C, Gunawardena A. Remodelling of lace plant leaves: antioxidants and ROS are key regulators of programmed cell death. Planta. 2017;246:133-147 pubmed publisher
Sultan L, Mileshina D, Grewe F, Rolle K, Abudraham S, Głodowicz P, et al. The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016;28:2805-2829 pubmed
Yin G, Whelan J, Wu S, Zhou J, Chen B, Chen X, et al. Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS ONE. 2016;11:e0148013 pubmed publisher
product information
Product number :
AS09 501
Product name :
Cat Catalase (peroxisomal marker)
Quantity :
50 µl
List Price :
302 USD
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
Immunogen :
KLH-conjugated peptide chosen from know plant catalase sequences including Arabidopsis thaliana isoforms: catalase-1 (Q96528, At1g20630), catalase-2 (P25819, At4g35090), catalase-3 (Q42547, At1g20620);
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Tested applications :
Immunogold (IG), Immunolocalization (IL), Immunoprecipitation (IP), Western blot (WB)
Recommended dilutions :
1: 500 (IG), 1: 25 - 1: 500 (IL), 2 µg (IP), 1 : 1000 (WB)
Expected apparent MW :
57 55 kDa
Confirmed reactivity :
Arabidopsis thaliana, Aponogeton madagascariensis, Brassica napus, Brassica oleracea, Hordeum vulgare, Lathyrus sativus, Lupinus albus, Lupinus luteus, Moniliophthora perniciosa, Musa acuminate, Musa paradisiaca L., Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Paulownia tomentosa, Picrorhiza kurroa, Pisum sativum, Plumbago zeylanica, Setaria italica L. P. Beauv, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera
Predicted reactivity :
Avicennia marina, Betula pendula, Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa subsp. pekinensis, Citrus sp., Citrus clementina, Citrus maxima, Camellia sinensis, Cucumis sativus, Cucurbita maxima, Cucurbita moschata, Elaeis guineensis var. tenera, Eucalyptus grandis, Fragaria ananassa, Glycine max, Gossypium mexicanum, Helianthus annus, Hibiscus cannabinus, Litchi chinensis, Lupinus albus, Manihot esculenta, Marchantia polymorpha, Morus notabilis, Nicotiana tabacum, Paenibacillus sp., Pinus pinea, Populus albaxtremula, Populus jackii, Prunus persica, Raphanus sativus, Saccharum officinarum, Sesamum indicum, Vicia faba
Not reactive in :
Chlamydomonas reinhardtii
Additional information :
To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.To decrease background signal this antibody needs to be incubated in PBS-T NOT TBS-T. For reference, check image in application example below.
Related products :
Plant protein extraction bufferSecondary antibodies
Selected references :
Tokarz et al. (2021). Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22(2):685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Li et al. (2021) Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021 Feb 1;8(1):31. doi: 10.1038/s41438-021-00470-w. PMID: 33518707; PMCID: PMC7848011.Wilmowicz et al (2021) EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers. International Journal of Molecular Sciences. 2021; 22(6):3001. https://doi.org/10.3390/ijms22063001Bapatla et al. (2021). Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum). Plants 10, no. 5: 987. https://doi.org/10.3390/plants10050987Adamiec et al. (2021). Fatty acid composition and cpDNA content in Arabidopsis thaliana mutants deprived of EGY1 protease. PHOTOSYNTHETICA 59 (4): 633-639, 2021, DOI 10.32615/ps.2021.053
Background :
Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
This antibody is recognizing all three isoforms of Arabidopsis thaliana catalase. Catalase-2 is a main isoform expressed in leaf tissue and localized to peroxisomes. This antibody contains 0.1 % ProClin.
UniProt number :
Q96528 , P25819 , Q42547
TAIR number :
AT4G35090
Commodity Code :
38220000
Our Category :
Peroxisomal marker
Research area :
Cell Biology
more info or order :
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
https://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.