This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
V-ATPase, D Vacuolar H+-ATPase subunit D
catalog :
AS09 497
quantity :
100 µl
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, ELISA
product information
Product number :
AS09 497
Product name :
V-ATPase, D Vacuolar H+-ATPase subunit D
Immunogen :
KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana V-ATPase subunit D UniProt: Q9XGM1, TAIR: At3g58730
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
100 µl
Tested applications :
ELISA (ELISA), Western blot (WB)
Related products :
collection of antibodies to vacuolar proteins AS09 466 Anti-V-ATPase subunit a vacuolar H+ ATPase, subunit a, rabbit antibodiesAS09 467 Anti-V-ATPase subunit A vacuolar H+-ATPase, rabbit antibodiesAS09 503 Anti-V-ATPase, B vacuolar ATP synthase subunit beta, rabbit antibodiesAS09 468 Anti-V-ATPase subunit c vacuolar H+-ATPase, subunit c (16 kDa) , rabbit antibodiesASS09 497 Anti- V-ATPase subunit D V-type proton ATPase subunit D, rabbit antibodiesAS07 213 Anti-V-ATPase subunit E of tonoplast H+ATPase, rabbit antibodiesAS09 499 Anti-V-ATPase subunit H V-type proton ATPase subunit H, rabbit antibodiesPlant protein extraction buffer
Recommended dilutions :
1 : 8000 (ELISA), 1 : 2000 (WB)
Expected apparent MW :
29 29 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Sorghum bicolor, Zea mays Species of your interest not listed? Contact us
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known
Additional information :
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Background :
V-ATPase is an enzyme responsible for acidification of intracellular compartments. Subunit D is the peripheral subunit of V1 complex. Alternative name: Vacuolar proton pump subunit D, vacuolar H(+)-ATPase subunit D
Reconstitution :
For reconstitution add 100 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
UniProt number :
Q9XGM1
TAIR number :
AT3G58730
Research area :
Arabidopsis thaliana
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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