antibody

TIP1;1, TIP1;2 tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 493

100 µl

polyclonal

domestic rabbit

nonconjugated

western blot, ELISA

TIP1;1, TIP1;2 tonoplast intrinistic protein 1-1, 1-2 (gamma)

KLH-conjugated synthetic peptide conserved in Raphanus sativus TIP1;1 and TIP1;2 (protein accesion number available in Suga et al. 2001). Peptide is also conserved in Arabidopsis thaliana TIP1-1 P25818, At2g36830, TIP1-2 Q41963, At3g26520

Rabbit

Polyclonal

Serum

Lyophilized

100 µl

ELISA (ELISA), Western blot (WB)

collection of antibodies to tonoplast proteins AS09 492 Anti-TIP1;1, TIP1;2 tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit anitbodiesAS09 494 Anti-TIP2;1 (delta) Delta-VM23, rabbit anitbodiesAS09 510 Anti-TIP2;1 tonoplast intrinistic protein 2-1, rabbit anitbodiesAS09 511 Anti-TIP2;2 tonoplast intrinistic protein 2-2 (C-terminal), rabbit anitbodiesAS09 493P TIP1;1, TIP1;2 tonoplast intrinistic protein 1-1, 1-2 (gamma) blocking peptidePlant protein extraction bufferSecondary antibodies

1 : 8000 (ELISA), 1 : 1000 (WB)

25,8 23 kDa

Arabidopsis thaliana, Raphanus sativus

Brassica napus, Gossypium hirsutum, Hordeum vulgare, Oryza sativa, Populus trichocarpa, Ricinus communisSpecies of your interest not listed? Contact us

No confirmed exceptions from predicted reactivity are currently known

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.Suga et al. (2001). Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporings in radish organs. Planta 212:294-304.

TIPs belong to MIP/aquaporin protein family. Alternative names: Aquaporin TIP1-1,gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, tonoplast intrinsic protein, root-specific RB7, gamma-tonoplast intrinsic protein 2, gamma-TIP2, salt stress-induced tonoplast intrinsic protein

For reconstitution add 100 µl of sterile water

Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

P25818 , Q41963

AT2G36830

Arabidopsis thaliana