This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
PIP2;1, PIP2;2, PIP2;3 Plasma membrane intrinistic protein 2-1,2-2,2-3
catalog :
AS09 491
quantity :
100 µl
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, ELISA
citations: 2
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product information
Product number :
AS09 491
Product name :
PIP2;1, PIP2;2, PIP2;3 Plasma membrane intrinistic protein 2-1,2-2,2-3
Immunogen :
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2 proteins: AtPIP2-1, At3g53420 , AtPIP2-2 P43287, At2g37170 , AtPIP2-3 P30302, At2g37180
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
100 µl
Tested applications :
ELISA (ELISA), Western blot (WB)
Related products :
collection of antibodies to plasma membrane proteins AS09 488 Anti-PIP2;1 aquaporin PIP2;1, rabbit antibodiesAS09 489 Anti-PIP1;1, PIP1;2, PIP1;3 aquaporins, rabbit antibodiesAS09 490 Anti-PIP2;2 plasma membrane aquaporin 2b, rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Recommended dilutions :
1 : 8000 (ELISA), 1 : 1000 (WB)
Expected apparent MW :
30,4 28 (PIP2-1,PIP2-2,PIP2-3) kDa
Confirmed reactivity :
Arabidopsis thaliana, Camelina sativa, Gromphadorhina coquereliana, Raphanus sativus
Predicted reactivity :
Brassica napus, Cucumis sativus, Glycine hispida, Gossypium hirsutum, Hedychium coronarium, Mimosa saman, Nicotiana glauca, Petunia hybrida, Pisum sativum, Ricinus communis, Populus tremula x Populus tremloides, Physcomitrium patens Species of your interest not listed? Contact us
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known
Additional information :
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
Selected references :
Carmona-Salazar et al. (2021). Plasma and Vacuolar Membrane Sphingolipidomes: Composition and Insights on The Role of Main Molecular Species. Plant Physiol. 2021 Feb 11:kiab064. doi: 10.1093/plphys/kiab064. Epub ahead of print. PMID: 33570616.Cano-Ramirez et al. (2021) M. Plasma Membrane Fluidity: An Environment Thermal Detector in Plants. Cells. 2021 Oct 17;10(10):2778. doi: 10.3390/cells10102778. PMID: 34685758; PMCID: PMC8535034.Hyun-Sung et al. (2019). NaCl-induced CsRCI2E and CsRCI2F interact with aquaporin CsPIP2;1 to reduce water transport in Camelina sativa L. Biochemical and Biophysical Research Communications,Available online 4 April 2019.Chowanski et al. (2015). Cold induced changes in lipid, protein and carbohydrate levels in the tropical insect Gromphadorhina coquereliana. Comp Biochem Physiol A Mol Integr Physiol. 2015 May;183:57-63. doi: 10.1016/j.cbpa.2015.01.007. Epub 2015 Jan 23.
Background :
PIP2;2 is a plasma membrane aquaporin. Alternative names of isoforms: aquaporin PIP2-1, plasma membrane intrinsic protein 2a, PIP2a, aquaporin PIP2-2, plasma membrane intrinsic protein 2b, PIP2b, TMP2b, Aquaporin PIP2-3, plasma membrane intrinsic protein 2c, PIP2c, TMP2C, RD28-PIP, water stress-induced tonoplast intrinsic protein, (WSII-TIP)
Reconstitution :
For reconstitution add 100 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
UniProt number :
P43286 , P43287 , P30302
TAIR number :
AT3G53420
Research area :
Arabidopsis thaliana
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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