antibody

PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) Aquaporins

AS09 487

50 µg

polyclonal

rabbit

nonconjugated

PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) Aquaporins

synthetic peptide conserved in Arabidopsis thaliana: PIP1;1 P61837,At3g61430 PIP1;2 Q06611,At2g45960 PIP1;3 Q08733,At1g01620 , PIP1;4 Q39196, At4g00430, PIP1;5 Q8LAA6 At4g23400

Rabbit

Polyclonal

Affinity purified serum in PBS, pH 7.4

Lyophilized

50 µg

Western blot (WB)

collection of antibodies to plasma membrane proteins AS09 487-ALP Anti-PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) aquaporins, ALP-conjugated (40 µg)AS09 487-HRP Anti-PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) aquaporins, HRP-conjugated (40 µg) AS09 488 Anti-PIP2;1 aquaporin PIP2;1, rabbit antibodiesAS09 489 Anti-PIP1;1, PIP1;2, PIP1;3 aquaporins, rabbit antibodiesAS09 490 Anti-PIP2;2 plasma membrane aquaporin 2b, rabbit antibodiesAS09 491 Anti-PIP2;1,PIP2;2,PIP2;3 plasma membrane intrinistic protein 2-1,2-2, 2-3, rabbit antibodiesPlant protein extraction bufferSecondary antibodies

1 : 1000 (WB)

30.68 28 kDa

Arabidopsis thaliana, Brassica sp., Jatropha curcas L. cv. "Biji Jarak ", Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea

Brassica sp., Hordeum vulgare, Juglans regia, Oryza sativa, Populus tremula, Triticum aestivum, Vicia faba

Fragaria sp.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Lopez et al. (2013). Aquaporins And Leaf Hydraulics, Poplar Sheds New Light. Plant Cell Physiol. Sep 20. Jang et al. (2013). Twoaquaporins of Jatropha are regulated differentially during drought stress and subsequent recovery. J Plant Physiol. March 25.

24058149, 23537705

PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Q06611 , Q08733 , Q39196

AT3G61430