MTP1 vacuolar Zn2+/H+ antiporter | Agrisera AS09 485 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Agrisera

product type :

antibody

product name :

MTP1 vacuolar Zn2+/H+ antiporter

catalog :

AS09 485

quantity :

100 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, ELISA

citations: 1

Reference
Vera Estrella R, Gómez Méndez M, Amezcua Romero J, Barkla B, Rosas Santiago P, Pantoja O. Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017;246:433-451 pubmed publisher

product information

Product number :

AS09 485

Product name :

MTP1 vacuolar Zn2+/H+ antiporter

Immunogen :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MTP1 UniProt:Q9ZT63, TAIR:At2g46800

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

100 µl

Tested applications :

ELISA (ELISA), Western blot (WB)

Related products :

collection of antibodies to plant tonoplast membrane proteinsAS09 474 Anti-AtMTP4 metal tolerance protein B, rabbit antibodiesPlant protein extraction bufferSecondary antibodies

Recommended dilutions :

1 : 8000 (ELISA), 1 : 1000 (WB)

Expected apparent MW :

43.8 43 kDa (Arabidopsis thaliana)

Confirmed reactivity :

Arabidopsis thaliana, Nicotiana tabacum

Predicted reactivity :

Brassica sp. , Noccaea fendleri, Thlaspi caerulescens Species of your interest not listed? Contact us

Not reactive in :

Hordeum vulgare, Solanaceae sp.

Additional information :

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel

Selected references :

Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1.Kawachi et al. (2008). Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity. J.Biol. Chem. 13:8374-8383.Kobae et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 12:1749-1758.

Background :

MTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved in sequestering of zinc from cytoplasm into vacuole in conditions of its excess. Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 metal tolerance protein 1

Reconstitution :

For reconstitution add 100 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.MTP1 protein is of low abundance in plant tissues.

UniProt number :

Q9ZT63

TAIR number :

AT2G46800

Research area :

Arabidopsis thaliana