product summary
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company name :
Agrisera
product type :
antibody
product name :
Na+/H+ antiporter, sodium/hydrogen exchanger
catalog :
AS09 484
quantity :
50 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, ELISA
more info or order :
citations: 4
| Reference |
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product information
Product number :
AS09 484
Product name :
Na+/H+ antiporter, sodium/hydrogen exchanger
Immunogen :
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NHX protein UniProt: Q68KI4, TAIR: At5g27150; chosen peptide is perfectly confirmed in AtNHX1 UniProt: Q0WVZ5, partially in AtNHX2, UniProt: Q56XP4, and not conserved in AtNHX3, UniProt: Q84WG1 and AtNHX4, UniProt:Q8S397 isoforms
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
ELISA (ELISA), Western blot (WB)
Related products :
collection of antibodies to tonoplast proteins Plant protein extraction bufferSecondary antibodies
Recommended dilutions :
1 : 8000 (ELISA), 1 : 1000 (WB)
Expected apparent MW :
59.5 45 kDa
Confirmed reactivity :
Arabidopsis thaliana, Kandelia obovata, Solanum lycopersicum
Predicted reactivity :
Gossypium hirsutum, Oryza sativa, Populus euphratica, Ricinus communis, Zea mays, Vitis vinifera
Not reactive in :
Mangrove plants, Nicotiana benthamiana, sp. Avicennia,
Selected references :
Guo et al. (2018). Molecular Characterization of a Tonoplast Na+/H+ Antiporter from Iris Lactea. Preprints 2018, 2018090557 (doi: 10.20944/preprints201809.0557.v1). Kumari et al. (2017), Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis. Front Plant Sci. 2017 Jan 6;7:2027. doi: 10.3389/fpls.2016.02027. Chen et al. (2013). Nitric Oxide Mediates Root K+/Na+ Balance in a Mangrove Plant, Kandelia obovata, by Enhancing the Expression of AKT1-Type K+ Channel and Na+/H+ Antiporter under High Salinity. 8(8): e71543. doi:10.1371/journal.pone.0071543.
Pubmed ID's :
28111589
Background :
Na(+)/H(+) exchanger 1 protein is involved in exchange of protons for cations such as sodium and potassium across membraes. Localized in tonoplast, possibily also to ER and Golgi. Alternative name: NHE-1, Na(+)/H(+) exchanger 1
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Protocol for vacuolar membrane isolation can be found here.
UniProt number :
Q68KI4
TAIR number :
AT5G27150
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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