TIP1 Tonoplast intrinsic protein 1-1 | Agrisera AS09 482 product information

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product summary

company name :

Agrisera

product type :

antibody

product name :

TIP1 Tonoplast intrinsic protein 1-1

catalog :

AS09 482

quantity :

100 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, ELISA

citations: 1

Reference
Kwon Y, Shen J, Lee M, Geem K, Jiang L, Hwang I. AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking. Proc Natl Acad Sci U S A. 2018;115:E1675-E1683 pubmed publisher

product information

Product number :

AS09 482

Product name :

TIP1 Tonoplast intrinsic protein 1-1

Immunogen :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana UniProt: P25818,TAIR:At2g36830

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

100 µl

Tested applications :

ELISA (ELISA), Western blot (WB)

Related products :

collection of antibodies to tonoplast proteins AS09 493 Anti-TIP1;1, TIP1;2 tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodiesAS09 494 Anti-TIP2;1 (delta) Delta-VM23, rabbit antibdiesAS09 510 Anti-TIP2;1 tonoplast intrinistic protein 2-1, rabbit antibodiesAS09 511 Anti-TIP2;2 tonoplast intrinistic protein 2-2 (C-terminal), rabbit antibodiesPlant protein extraction bufferSecondary antibodies

Recommended dilutions :

1 : 8000 (ELISA), 1 : 2000 (WB)

Expected apparent MW :

25.6 24 kDa (Arabidopsis thaliana)

Confirmed reactivity :

Arabidopsis thaliana

Predicted reactivity :

Brassica oleracea, Gossypium mexicanum, Oryza sativa, Pisum sativum, Picea abies, Popoulus trichocarpa, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known

Additional information :

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Selected references :

Kwon et al. (2018). AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1675-E1683. doi: 10.1073/pnas.1717204115.

Background :

TIP1;1 is a water channel protein needed for the transport of water, diffusion of amino acids and peptides. Alternative name: Tonoplast intrinsic protein 1-1, gamma-tonoplast intrinsic protein, Gamma-TIP, aquaporin TIP, Tonoplast intrinsic protein, root-specific RB7

Reconstitution :

For reconstitution add 100 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.TIP1;1 usually shows a faint band of dimeric form at 42 kDa in addition to the major monomoer band of 23 kDa even in the presence of SDS.

UniProt number :

P25818

TAIR number :

AT2G36830

Research area :

Arabidopsis thaliana