BiP Lumenal-binding protein (rabbit antibody) | Agrisera AS09 481 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

BiP Lumenal-binding protein (rabbit antibody)

catalog :

AS09 481

quantity :

50 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, ELISA, immunocytochemistry

more info or order :

Agrisera product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; thale cress; 1:2000; fig 4	Jozwiak A, Gutkowska M, Gawarecka K, Surmacz L, Buczkowska A, Lichocka M, et al. POLYPRENOL REDUCTASE2 Deficiency Is Lethal in Arabidopsis Due to Male Sterility. Plant Cell. 2015;27:3336-53 pubmed publisher
	Wang Q, Li Y, Ishikawa K, Kosami K, Uno K, Nagawa S, et al. Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018;115:E11551-E11560 pubmed publisher
	Mares J, Gramacho K, Santos E, da Silva Santiago A, Santana J, de Sousa A, et al. Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches' broom disease in cacao. BMC Microbiol. 2017;17:176 pubmed publisher
	Buxa S, Degola F, Polizzotto R, De Marco F, Loschi A, Kogel K, et al. Phytoplasma infection in tomato is associated with re-organization of plasma membrane, ER stacks, and actin filaments in sieve elements. Front Plant Sci. 2015;6:650 pubmed publisher
	Wilson S, Ho Y, Lampugnani E, van de Meene A, Bain M, Bacic A, et al. Determining the subcellular location of synthesis and assembly of the cell wall polysaccharide (1,3; 1,4)-β-D-glucan in grasses. Plant Cell. 2015;27:754-71 pubmed publisher
	Lopez Huertas E, del Rio L. Characterization of antioxidant enzymes and peroxisomes of olive (Olea europaea L.) fruits. J Plant Physiol. 2014;171:1463-71 pubmed publisher
	Tanaka H, Nodzyłski T, Kitakura S, Feraru M, Sasabe M, Ishikawa T, et al. BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in Arabidopsis. Plant Cell Physiol. 2014;55:737-49 pubmed publisher
	Sikorskaite S, Rajamäki M, Baniulis D, Stanys V, Valkonen J. Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families. Plant Methods. 2013;9:31 pubmed publisher
	Tanaka H, Kitakura S, Rakusová H, Uemura T, Feraru M, De Rycke R, et al. Cell polarity and patterning by PIN trafficking through early endosomal compartments in Arabidopsis thaliana. PLoS Genet. 2013;9:e1003540 pubmed publisher

product information

Product number :

AS09 481

Product name :

BiP Lumenal-binding protein (rabbit antibody)

Immunogen :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080 Q8H1B3

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4

Format :

Lyophilized

Quantity :

50 µg

Tested applications :

ELISA (ELISA), Immunofluorescence (IF), Immunogold (IG), Western blot (WB)

Related products :

AS09 615 BiP2 lumenal-binding protein 2, goat antibodyAS09 614 BiP2 lumenal-binding protein 2, hen antibodyAS09 481PRE BiP lumenal-binding protein, pre-immune serumantibodies to plant endomembrane system proteins Plant protein extraction bufferSecondary antibodies

Recommended dilutions :

1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)

Expected apparent MW :

73.5 80 kDa

Confirmed reactivity :

Arabidopsis thaliana, Brassica napus, Chara australis R.Br, Chlamydomonas reinhardtii, Cucumis sativus, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Oryza sativa, Picea abies, Physcomitrella patens, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays

Predicted reactivity :

Arabis alpina, Capsella rubella, Capsicum annuum, Citrus clementina, Citrus sinsensis, Eucalyptus grandis, Glycine max, Hordeum vulgare, Isatis tincorina, Prunus persica, Triticum aestivium, Picea sitcHensis, Populus trichocarpa, Ricinus comminus, Vitis vinifera

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. This antibody has so far not worked in IP.

Selected references :

Pertl-Obermeyer et al. (2018). Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS One. 2018 Aug 29;13(8):e0201480. doi: 10.1371/journal.pone.0201480. ï»¿ï»¿ï»¿Qiao et al. (2018). Two Crinivirus-Conserved Small Proteins, P5 and P9, Are Indispensable for Efficient Lettuce infectious yellows virus Infectivity in Plants. Viruses. 2018 Aug 28;10(9). pii: E459. doi: 10.3390/v10090459. Mares et al. (2017). Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches' broom disease in cacao. BMC Microbiol. 2017 Aug 17;17(1):176. doi: 10.1186/s12866-017-1085-4. GelovÃ¡ et al. (2017). Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis. J Exp Bot. 2017 Dec 23. doi: 10.1093/jxb/erx426.Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114. Lomin et al. (2017). Studies of cytokinin receptorâ€“phosphotransmitter interaction provide evidences for the initiation of cytokinin signalling in the endoplasmic reticulum. Functional Plant Biology, CSIRO Publications. (Nicotiana benthamiana, western blot) Zhang et al. (2017). Control of secondary cell wall patterning involves xylan deacetylation by a GDSL esterase. Nat Plants. 2017 Mar 3;3:17017. doi: 10.1038/nplants.2017.17. (Oryza sativa, immunolocalization, western blot)Je et al. (2016). Signaling from maize organ primordia via FASCIATED EAR3 regulates stem cell proliferation and yield traits. Nat Genet. 2016 Jul;48(7):785-91. doi: 10.1038/ng.3567. Epub 2016 May 16.

Pubmed ID's :

30157181, 30154314, 28818052, 29294075, 28784794, 28260782, 27182966

Background :

BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt number :

Q39043

TAIR number :

AT5G42020, AT5G42020

more info or order :

Agrisera product webpage