PDR8 ABC transporter G family member 36 | Agrisera AS09 471 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Agrisera

product type :

antibody

product name :

PDR8 ABC transporter G family member 36

catalog :

AS09 471

quantity :

100 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot, ELISA

citations: 1

Reference
Campe R, Langenbach C, Leissing F, Popescu G, Popescu S, Goellner K, et al. ABC transporter PEN3/PDR8/ABCG36 interacts with calmodulin that, like PEN3, is required for Arabidopsis nonhost resistance. New Phytol. 2016;209:294-306 pubmed publisher

product information

Product number :

AS09 471

Product name :

PDR8 ABC transporter G family member 36

Immunogen :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PDR8, Q9XIE2, At1g59870

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

100 µl

Tested applications :

ELISA (ELISA), Western blot (WB)

Related products :

collection of antibodies to plant plasma membrane proteinsPlant protein extraction bufferSecondary antibodies

Recommended dilutions :

1 : 8000 (ELISA), 1 : 1000 (WB)

Expected apparent MW :

165 150 kDa (Arabidopsis thaliana)

Confirmed reactivity :

Arabidopsis thaliana

Predicted reactivity :

Arabidopsis thaliana

Not reactive in :

Triticum sp.

Additional information :

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Protocol for preparation of plant plasma membranes can be found under "more information" tab. Additoinal 160 kDa cross-reacting band is detected when working with chloroplast fractions.

Selected references :

Campe at al. (2015). ABC transporter PEN3/PDR8/ABCG36 interacts with calmodulin that, like PEN3, is required for Arabidopsis nonhost resistance. New Phytol. 2015 Aug 28. doi: 10.1111/nph.13582.Yoshihiro et al. (2006). Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection. Plant Cell Physiol. 47:309-318.

Background :

PDR8 protein is a key factor which controls the extent of cell death in defence response. Alternative names: ABC transporter ABCG.36, AtABCG36, pleiotropic drug resistance protein 8, protein PENETRATION 3

Reconstitution :

For reconstitution add 100 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

UniProt number :

Q9XIE2

TAIR number :

AT1G59870

Research area :

Arabidopsis thaliana