PIP2;7 Plasma membrane aquaporin, N-terminal | Agrisera AS09 469 product information

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product summary

company name :

Agrisera

product type :

antibody

product name :

PIP2;7 Plasma membrane aquaporin, N-terminal

catalog :

AS09 469

quantity :

100 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, ELISA

citations: 1

Reference
Pou A, Jeanguenin L, Milhiet T, Batoko H, Chaumont F, Hachez C. Salinity-mediated transcriptional and post-translational regulation of the Arabidopsis aquaporin PIP2;7. Plant Mol Biol. 2016;92:731-744 pubmed

product information

Product number :

AS09 469

Product name :

PIP2;7 Plasma membrane aquaporin, N-terminal

Immunogen :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2;7,P93004, At4g35100

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

100 µl

Tested applications :

ELISA (ELISA), Western blot (WB)

Related products :

AS09 488 Anti-PIP2;1 aquaporin PIP2;1, rabbit antibodiesAS09 490 Anti-PIP2;2 plasma membrane aquaporin 2b, rabbit antibodiesAS09 491 Anti-PIP2;1,PIP2;2,PIP2;3 plasma membrane intrinistic protein 2-1,2-2, 2-3, rabbit antibodiesPlant protein extraction buffer

Recommended dilutions :

1 : 8000 (ELISA), 1 : 2000 (WB)

Expected apparent MW :

29.7 30.5 kDa (Arabidopsis thaliana)

Confirmed reactivity :

Arabidopsis thaliana, Jatropha curcas L. cv. Biji Jarak

Predicted reactivity :

Brassica oleracea, Juglans regia, Populus tremulax Populus tremuloides, Spinacia oleracea, Vitis viniferaSpecies of your interest not listed? Contact us

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known

Additional information :

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Theoretically this antibody can also react with PIP2;8 but it has not been confirmed experimentally.

Selected references :

Pou et al. (2016). Salinity-mediated transcriptional and post-translational regulation of the Arabidopsis aquaporin PIP2;7. Plant Mol Biol. 2016 Sep 26.Hachez et al. (2014). The Arabidopsis Abiotic Stress-Induced TSPO-Related Protein Reduces Cell-Surface Expression of the Aquaporin PIP2;7 through Protein-Protein Interactions and Autophagic Degradation. Plant Cell. 2014 Dec;26(12):4974-90. doi: 10.1105/tpc.114.134080. Epub 2014 Dec 23.Jang et al. (2013). Twoaquaporins of Jatropha are regulated differentially during drought stress and subsequent recovery. J Plant Physiol. March 25.

Background :

Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane. Alternative names: plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a

Reconstitution :

For reconstitution add 100 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of plasma membranes are used, one µg load per well should be sufficient.This antibody has a potential to work in immunolocalization studies, as it is recognizing N-terminall part of the sequence, which is exposed to the cytoplasm.

UniProt number :

P93004

TAIR number :

AT4G35100

Research area :

Arabidopsis thaliana