V-ATPase, c Vacuolar H+-ATPase, subunit c (16 kDa) | Agrisera AS09 468 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

V-ATPase, c Vacuolar H+-ATPase, subunit c (16 kDa)

catalog :

AS09 468

quantity :

50 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

product information

Product number :

AS09 468

Product name :

V-ATPase, c Vacuolar H+-ATPase, subunit c (16 kDa)

Immunogen :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit c UniProt: Q6IDA4

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4

Format :

Lyophilized

Quantity :

50 µg

Tested applications :

Western blot (WB)

Related products :

Collection of antibodies to vacuolar proteins AS09 467 anti-V-ATPase subunit A vacuolar H+-ATPaseAS09 503 anti-V-ATPase, B vacuolar ATP synthase subunit betaASS09 497 anti- V-ATPase subunit D V-type proton ATPase subunit DAS07 213 anti-V-ATPase subunit E of tonoplast H+ATPaseAS09 499 anti-V-ATPase subunit H V-type proton ATPase subunit HPlant protein extraction bufferSecondary antibodies

Recommended dilutions :

1 : 1000 (WB)

Expected apparent MW :

16 16 kDa (Arabidopsis thaliana)

Confirmed reactivity :

Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana tabacum

Predicted reactivity :

dictos including: Cucumis sativus, Gossypium mexicanum, Phaseolus aureus, Raphanus sativus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa

Not reactive in :

Algae

Additional information :

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Selected references :

Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1. Barkla et al. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016 May 10;16(1):110. doi: 10.1186/s12870-016-0797-1.

Pubmed ID's :

28455771, 27160145

Background :

V-ATPase c subunit is located in vacuole and is involved in ATP synthesis cuopled proton transport. This protein is coded by ATVHA-C3 gene. Alternative names: AT4g34720/T4L20_300

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

Subunit c is one of most hydrophobic proteins (can be dissolved in organic solvent such as a mixture of chloroform/methanol solution). It is prone to aggregation even in the presence of SDS. Therefore, before loading on the gel membrane fractions should be incubated in buffer containing 2 % SDS at 60° or 70°C for 10 min or at 25°C for 30 min.

UniProt number :

Q6IDA4

TAIR number :

AT2G16510

more info or order :

Agrisera product webpage