antibody

V-ATPase, A Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG)

AS09 467

100 µl

polyclonal

domestic rabbit

nonconjugated

western blot, ELISA

V-ATPase, A Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG)

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit A, O23654, At1g78900

Rabbit

Polyclonal

Ammonium sulfate purified, total IgG.

Lyophilized

100 µl

ELISA (ELISA), Western blot (WB)

Collection of antibodies to other vacuolar membrane proteinsPlant protein extraction bufferSecondary antibodies

1 : 8000 (ELISA), 1 : 2000 (WB)

68.8 70 kDa (Arabidopsis thaliana)

Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana tabacum

Chlamydomonas reinhardtii, Brassica napus, Cucumis sativus, Gossypium mexicanum, Hordeum vulgare, Oryza sativa, Ostreococcus lucimarinus, Phaseolus aureus, Populus balsamifera, Physcomitrium patens, Solanum lycopersicon, Triticum aestivum, Zea mays Species of your interest not listed? Contact us

Thermotoga neapolitana

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1.Barkla et al. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016 May 10;16(1):110. doi: 10.1186/s12870-016-0797-1.Yoshihiro et al. (2006) Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells. Cells. Biosci.Biotechnol. Biochem. 70: 980-987.

V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit

For reconstitution add 100 µl of sterile water

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube label.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of plant vacuolar membranes can be found here.

O23654

AT1G78900

Arabidopsis thaliana