This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
V-ATPase, a Vacuolar H+ ATPase, subunit a
catalog :
AS09 466
quantity :
100 µl
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, ELISA
citations: 1
product information
Product number :
AS09 466
Product name :
V-ATPase, a Vacuolar H+ ATPase, subunit a
Immunogen :
KLH-cougted synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit a, Q9SJT7, At2g21410
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
100 µl
Tested applications :
ELISA (ELISA), Western blot (WB)
Related products :
collection of antibodies to vacuolar proteins AS09 467 Anti-V-ATPase subunit A vacuolar H+-ATPase, rabbit antibodiesAS09 503 Anti-V-ATPase, B vacuolar ATP synthase subunit beta, rabbit antibodiesAS09 468 Anti-V-ATPase subunit c vacuolar H+-ATPase, subunit c (16 kDa), rabbit antibodiesAS09 497 Anti- V-ATPase subunit D V-type proton ATPase subunit D, rabbit antibodiesAS07 213 Anti-V-ATPase subunit E of tonoplast H+ATPase, rabbit antibodiesAS09 499 Anti-V-ATPase subunit H V-type proton ATPase subunit H, rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Recommended dilutions :
1 : 8000 (ELISA), 1 : 2000 (WB)
Expected apparent MW :
93 100 kDa (Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Cucumis sativus, Oryza sativa
Predicted reactivity :
Chlamydomonas reainhardtii, Physcomitrium patens, Populus balsamifera, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Not reactive in :
Thermotoga neapolitana
Additional information :
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Selected references :
Xing et al. (2016). Proteome Profile of Starch Granules Purified from Rice (Oryza sativa) Endosperm. PLoS One. 2016 Dec 19;11(12):e0168467. doi: 10.1371/journal.pone.0168467.Migocka et al. (2013). NO3 (-)/H(+) Antiport in the Tonoplast of Cucumber Root Cells Is Stimulated by Nitrate Supply: Evidence for a Reversible Nitrate-Induced Phosphorylation of Vacuolar NO3 (-)/H(+) Antiport.PLoS One. 2013 Sep 11;8(9):e73972. doi: 10.1371/journal.pone.0073972.Fumiyoshi et al. (2005). Novel type aquaporin SIPs, are mainly localized the ER membrane and show cell-specific expression in Arabidopsis thaliana. FEBS Lett. 579: 5814-58200.Yoshihiro et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 45: 1749-1758.
Background :
V-ATPase subunit a is coded by VHA-A2 gene. It has hydrogen ion transmembrane transporter activity. Alternative names: At2g21410/F3K23.17, putative vacuolar proton-ATPase subunit, V-ATPase subunit a (100 kDa subunit), VHA-a
Reconstitution :
For reconstitution add 100 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar membranes are used, one µg load per well should be sufficient.Protocol of isolation of plant vacuolar membranes can be found here.
UniProt number :
Q9SJT7
TAIR number :
AT2G21410
Research area :
Arabidopsis thaliana
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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