PsaD PSI-D subunit of photosystem I | Agrisera AS09 461 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

PsaD PSI-D subunit of photosystem I

catalog :

AS09 461

quantity :

50 µl

price :

394 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, immunoprecipitation

more info or order :

Agrisera product webpage

citations: 16

Reference
Pipitone R, Eicke S, Pfister B, Glauser G, Falconet D, Uwizeye C, et al. A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. elife. 2021;10: pubmed publisher
Kameo S, Aso M, Furukawa R, Matsumae R, Yokono M, Fujita T, et al. Substitution of deoxycholate with the amphiphilic polymer amphipol A8-35 improves the stability of large protein complexes during native electrophoresis. Plant Cell Physiol. 2021;: pubmed publisher
Tang Y, Gao C, Gao Y, Yang Y, Shi B, Yu J, et al. OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature. Dev Cell. 2020;53:272-286.e7 pubmed publisher
Xu D, Leister D, Kleine T. VENOSA4, a Human dNTPase SAMHD1 Homolog, Contributes to Chloroplast Development and Abiotic Stress Tolerance. Plant Physiol. 2020;182:721-729 pubmed publisher
Chen Y, Mao H, Wu N, Ma J, Yuan M, Zhang Z, et al. Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat. Int J Mol Sci. 2019;21: pubmed publisher
Storti M, Alboresi A, Gerotto C, Aro E, Finazzi G, Morosinotto T. Role of cyclic and pseudo-cyclic electron transport in response to dynamic light changes in Physcomitrella patens. Plant Cell Environ. 2019;42:1590-1602 pubmed publisher
Mao H, Chen M, Su Y, Wu N, Yuan M, Yuan S, et al. Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018;19: pubmed publisher
Li S, Tian Y, Wu K, Ye Y, Yu J, Zhang J, et al. Modulating plant growth-metabolism coordination for sustainable agriculture. Nature. 2018;560:595-600 pubmed publisher
Kong F, Burlacot A, Liang Y, Légeret B, Alseekh S, Brotman Y, et al. Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell. 2018;30:1824-1847 pubmed publisher
Nama S, Madireddi S, Yadav R, Subramanyam R. Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2019;139:387-400 pubmed publisher
Du Z, Lucker B, Zienkiewicz K, Miller T, Zienkiewicz A, Sears B, et al. Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018;30:447-465 pubmed publisher
Ge H, Fang L, Huang X, Wang J, Chen W, Liu Y, et al. Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017;16:1258-1274 pubmed publisher
Merry R, Jerrard J, Frebault J, Verhoeven A. A comparison of pine and spruce in recovery from winter stress; changes in recovery kinetics, and the abundance and phosphorylation status of photosynthetic proteins during winter. Tree Physiol. 2017;37:1239-1250 pubmed publisher
Schöttler M, Thiele W, Belkius K, Bergner S, Flügel C, Wittenberg G, et al. The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. 2017;68:1137-1155 pubmed publisher
Heinnickel M, Kim R, Wittkopp T, Yang W, Walters K, Herbert S, et al. Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016;113:2774-9 pubmed publisher
Fujii R, Yamano N, Hashimoto H, Misawa N, Ifuku K. Photoprotection vs. Photoinhibition of Photosystem II in Transplastomic Lettuce (Lactuca sativa) Dominantly Accumulating Astaxanthin. Plant Cell Physiol. 2016;57:1518-1529 pubmed

product information

Product number :

AS09 461

Product name :

PsaD PSI-D subunit of photosystem I

Quantity :

50 µl

List Price :

394 USD

Immunogen :

KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences including Arabidopsis thaliana PSI-D1 UniProt:Q9S7H1 , TAIR: At4g02770 and PSI-D2 UniProt: Q9SA56 , TAIR At1g03130 as well as Physcomitrella patens. The conservation in Chlamydomonas reinhardtii is high (14 of 16 aminoacids are identical).

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Clear-native PAGE (CN-PAGE), Immunoprecipitation (IP), Western blot (WB)

Recommended dilutions :

1: 10 000 (CN-PAGE), 1 : 1000 - 1: 5 000 (WB)

Expected apparent MW :

17.9 20 (for Arabidopsis thaliana)

Confirmed reactivity :

Arabidopsis thaliana, Chlamydomonas reinhardtii, Dioxoniella giordanoi (red alga), Hordeum vulgare, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Picea glauca, Pinus strobus, Oryza sativa, Physcomitrium patens, Spinacia oleracea, Synechocystis PCC 6803, Triticum aestivum, Triticale, Zea mays

Predicted reactivity :

Alge, Dicots, Catalpa bungei, Cucumis melo, Conifers, Cyanidioschyzon merolae, Bigelowiella natans, Nannochloropsis sp. , Phaeodactylum tricornutum, Phyla dulcis, Zosteria marina

Not reactive in :

Synechococcus elongatus sp. PCC 7942

Additional information :

This antibody is a replacement for former product, anti-PsaD AS04 046 Contains 0.1% ProClin.

Related products :

Plant protein extraction bufferSecondary antibodies

Selected references :

Ivanov et al. (2022) The decreased PG content of pgp1 inhibits PSI photochemistry and limits reaction center and light-harvesting polypeptide accumulation in response to cold acclimation. Planta 255, 36 (2022). https://doi.org/10.1007/s00425-022-03819-0Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Fattore et al. (2021). Acclimation of photosynthetic apparatus in the mesophilic red alga Dixoniella giordanoi. Physiol Plant. 2021 Nov;173(3):805-817. doi: 10.1111/ppl.13489. Epub 2021 Jul 5. PMID: 34171145; PMCID: PMC8596783.Chen et al. (2021)Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis,Plant Science,Volume 307,2021,110902,ISSN 0168-9452,https://doi.org/10.1016/j.plantsci.2021.110902. Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.

Background :

PsaD (PSI-D) is a core subunit of photosystem I highly conserved in all photosynthetic organisms (including bacteria with Fe-S type reaction centers). In eukaryots its encoded by 1 to 2 nuclear gene(s) and imported as a precursor into the chloroplast. In the thylakoid membrane it associates with PsaA and PsaB on the stromal site of the PSI core forming the Fd-docking site. PsaD is also required for the stable assembly of PsaC.

Reconstitution :

For reconstitution add 50 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

PsaD has frequently been used as a marker for intact PSI reaction centers.This product can be sold containing proclin if requested.

UniProt number :

Q9SA56 , Q9S7H1

TAIR number :

AT1G03130, AT4G02770

Commodity Code :

38220000

Our Category :

PSI (Photosystem I)

Research area :

Plant Biology, Photosynthesis

more info or order :

Agrisera product webpage