PEPC Phosphoenolpyruvate carboxylase | Agrisera AS09 458 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

PEPC Phosphoenolpyruvate carboxylase

catalog :

AS09 458

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 1

Reference
Ermakova M, López Calcagno P, Raines C, Furbank R, von Caemmerer S. Overexpression of the Rieske FeS protein of the Cytochrome b6f complex increases C4 photosynthesis in Setaria viridis. Commun Biol. 2019;2:314 pubmed publisher

product information

Product number :

AS09 458

Product name :

PEPC Phosphoenolpyruvate carboxylase

Immunogen :

KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4). For Zea mays, the peptide is converved in PEP1 and PEP4 isoforms.

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4

Format :

Lyophilized

Quantity :

50 µg

Tested applications :

Immunolocalization (IL), Western blot (WB)

Related products :

AS07 241 anti-PEPCK PEP carboxy kinase

Recommended dilutions :

1 : 500 (IL), 1 : 1000 (WB)

Expected apparent MW :

110 105 kDa

Confirmed reactivity :

Ananas comosus, Arabidopsis thaliana, Cenchrus ciliaris, Chloris gayana, Chromera velia, Hordeum vulgare, Jatropha curcas, Leptochloa fusca, Lupinus sp. , Megathyrsus maximus, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Panicum antidotale, Panicum coloratum, Pinus strobus, Saccharum spp. hybrid clone C91-301, Salsola lanata, Salsola laricifolia, Sorghum bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), Pinus strobus, Thalassiosira weissfloggi, Zea mays, Zostera muelleri

Predicted reactivity :

Cucumis sativus (PEPC1, PEPC2, PEPC3), Flaveria bidentis, Flaveria trinervia, Glycine max, Lupinus albus, Mammillaria thornberi, Manihot obovata, Medicago sativa, Morinda citrifolia, Nopalea gaumeri, Opuntia macbridei, Pachycereus pringlei, Saccharum spp, Solanum tuberosum, Spinacia oleracea, Streptanthus tortuosus, Pachycereus hollianus, Pisum sativa, Phaseolus vulgaris, Triticum aestivum, algae, diatoms: Thalassiosira pseudonana, other species: Salmonella sp., Schiedea hookeri, Shigella sp. Schiedea sarmentosa, Streptanthus farnsworthianus, Tacinga saxatilis,Yersinia sp. Vibrio sp.

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

Antibody can be also used following 2D gel electrophoresis.

Selected references :

Bassi et al. (2018). Nitrogen supply influences photosynthesis establishment along the sugarcane leaf. Sci Rep. 2018 Feb 2;8(1):2327. doi: 10.1038/s41598-018-20653-1. Sonawane et al. (2018). Shade compromises the photosynthetic efficiency of NADP-ME less than PEP-CK and NAD-ME C 4 grasses. J Exp. Botany, doi.org/10.1093/jxb/ery129. Wen et al. (2017). Possible involvement of phosphoenolpyruvate carboxylase and NAD-malic enzyme in response to drought stress. A case study: A succulent nature of the C4-NAD-ME type desert plant, Salsola lanata (Chenopodiaceae). Functional Plant Biology 44(12), DOI10.1071/FP16430 Jiang et al. (2017). Development of an Efficient Protein Extraction Method Compatible with LC-MS/MS for Proteome Mapping in Two Australian Seagrasses Zostera muelleri and Posidonia australis. Frontiers in Plant Science, doi: 10.3389/fpls.2017.01416. Liu et al. (2017). Plasma Membrane CRPK1-Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import to Fine-Tune CBF Signaling during Cold Response. Mol Cell. 2017 Apr 6;66(1):117-128.e5. doi: 10.1016/j.molcel.2017.02.016. Ribeiro et al. (2017). Increased sink strength offsets the inhibitory effect of sucrose on sugarcane photosynthesis. J Plant Physiol. 2017 Jan;208:61-69. doi: 10.1016/j.jplph.2016.11.005.Shen et al. (2016). The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y). 2016 Dec;9(1):20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.Ishikawa et al. (2016). NDH-Mediated Cyclic Electron Flow Around Photosystem I is Crucial for C4 Photosynthesis. Plant Cell Physiol. 2016 Aug 6. pii: pcw127. [Epub ahead of print] Shen et al. (2015). Overexpression of maize phosphoenolpyruvate carboxylase improves drought tolerance in rice by stabilization the function and structure of thylakoid membrane. Photosynthetica, September 2015, Volume 53, Issue 3, pp 436-446.Foley et. al (2015). Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches. BMC Plant Biology 2015, 15:106 doi:10.1186/s12870-015-0485-6.

Pubmed ID's :

29396510, 28344081, 27889522, 27164981, 27497446

Background :

PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.Please do not re-use this primary antibody solution. In case of cyanobacterial samples there will be no signal in your second incubation.

UniProt number :

Q9MAH0 , Q84VW9

TAIR number :

AT1G53310, AT3G14940

more info or order :

Agrisera product webpage