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product summary
company name :
Agrisera
product type :
antibody
product name :
GP Glycogen phosphorylase
catalog :
AS09 455
quantity :
50 µg
price :
302 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
citations: 2
| Published Application/Species/Sample/Dilution | Reference |
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product information
Product number :
AS09 455
Product name :
GP Glycogen phosphorylase
Quantity :
50 µg
List Price :
302 USD
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
Immunogen :
KLH-conjugated peptide derived from the sequence of human glycogen phosphorylase P11217
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Tested applications :
Immunoprecipitation (IP), Western blot (WB)
Recommended dilutions :
5 µg (IP), 1 : 2000 (WB)
Expected apparent MW :
97 kDa
Confirmed reactivity :
Human, Mouse, Rabbit,Rat, Trichomonas vaginalis G3
Predicted reactivity :
Atlantic Salmon, Bovine, Dog, Drosophila melanogaster, Hen, Horse, Pig, Zebrafish, Xenopus laevis
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Samples used to test this antibody were: muscle and liver homogenates, purified glycogen, This antiboy will recogize glycogen phosphorylase from both liver and muscle, It has been used in ICC on human primary myotubes
Related products :
Secondary antibodies
Selected references :
Mia et al. (2020). Differential Effects of REV-ERBa/b Agonism on Cardiac Gene Expression, Metabolism, and Contractile Function in a Mouse Model of Circadian Disruption. Am J Physiol Heart Circ Physiol. 2020 May 1. doi: 10.1152/ajpheart.00709.2019. Dandanell et al. (2016). Maintaining a clinical weight loss after intensive lifestyle intervention is the key to cardiometabolic health. pii: S1871-403X(16)30107-7. doi: 10.1016/j.orcp.2016.09.009.Bowker and Zhuang (2015). Relationship between water-holding capacity and protein denaturation in broiler breast meat. Poult Sci. 2015 May 25. pii: pev120.Zhu et al. (2011). High post-mortem temperature combined with rapid glycolysis induces phosphorylase denaturation and produces pale and exudative characteristics in broiler Pectoralis major muscles. Meat Science
Background :
Glycogen phosphorylase (EC 2.4.1.1) is an enzyme which is catalyzing the rate limiting step in the degradation of glycogen in animals by releasing glucose-1-phosphate from the terminal alpha1,4-glycosidic bond.
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
This antibody can detect GP in crude tissue homogenate in both, liver and muscle tissue and is able to immunoprecipiate GP.Glycogen phosphorylase is a good marker for determining the degree of postmortem protein denaturation in muscle. When glycogen phosphorylase denatures it shifts from the sarcoplasmic fraction of the muscle to the myofibrillar fraction of the muscle. This shift is detectable using SDS-PAGE and western blotting techniques. The antibody is being used to detect glycogen phosphorylase levels in both sarcoplasmic and myofibrillar protein extracts from chicken muscle.
UniProt number :
P11217
Commodity Code :
38220000
Our Category :
Other proteins
Research area :
Proteomics
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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