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Rubisco quantitation kit (Western blot)

AS09 409set

682 USD

Rubisco quantitation kit (Western blot)

Antibody set

Primary antibody: Rabbit, Secondary antibody: Goat

Polyclonal

1 x 50 µl of AS03 037, RbcL Rubisco large subunit, form I and form II (amount enough for 50-100 Western blots)1 x 100 µl of AS01 017S, Rubisco protein standard (0.15 pmoles / µl, amount enough for generation of standard curve in 34 assays (standard curve: 0.0625 pmoles, 0.125 pmoles, 0.25 pmoles)2 x 2 ml of AS08 300, Protein extraction buffer (amount enough for 48 isolations of plant material, using 500 µl 1 x PEB for 100 mg fresh weight) or 120 isolations of algal material (using 200 µl 1 x PEB for cell amounts corresponding to 4-10 µg total chlorophyll)2 x 10 µl of AS09 602, Goat anti-rabbit IgG (H&L), HRP conjugated (amount enough for 50-100 Western blots)

682 USD

Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Cynara cardunculus var altilis, Emiliana huxleyi, Euglena gracilis, Gonyaulax polyedra, Heterosigma akashiwo, Micromonas pusila, Porphyra sp. Spinacia oleracea, Synechococcus PCC 7942, Thalassiosira pseudonana

KLH-conjugated synthetic peptide was used to elicit anti-RbcL antibody. No baculovirus was used in production of this product.

Western blot (WB)

Western blot (WB)

RbcL antibody and protein standard: Please store at -20°C (6 months) or -80°C for long term storage(years). Please avoid freezing and thawing of reconstituted antibodies, make aliquots instead. PEB extraction buffer:Stable at RT for at least 1 month. For short-term storage please stoore (1 month) at 4°C and for long term storage (1 year) at -20°C.