product summary
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company name :
Agrisera
product type :
antibody
product name :
AO ascorbate oxidase, Biotin conjugated
catalog :
AS09 383
quantity :
10 mg
price :
401 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
biotin
application :
western blot, ELISA
more info or order :
product information
Product number :
AS09 383
Product name :
AO ascorbate oxidase, Biotin conjugated
Quantity :
10 mg
List Price :
401 USD
Immunogen :
Ascorbate oxidase purified from Cucurbita sp.
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Purified IgG in PBS pH 7.2.
Format :
Lyophilized
Tested applications :
ELISA (ELISA), Western blot (WB)
Recommended dilutions :
1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)
Expected apparent MW :
61 45 kDa (Cucurbita pepo)
Confirmed reactivity :
Cucurbita sp.
Predicted reactivity :
Glycne max, Oryza sativa Species of your interest not listed? Contact us
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
IgG concentration is 10 mg/ml, Biotin/IgG protein molar ratio is approximately 5,6, No foreign proteins are added
Related products :
Plant and algal protein extraction bufferSecondary antibodies
Background :
Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Reconstitution :
For reconstitution add 1 ml of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.
Additional information :
IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography, N-Hydroxysuccinimidobiotin is used for labelling of antibody
Commodity Code :
38220000
Our Category :
Plant Oxidative Stress
Research area :
Plant Biology, Enviromental stress
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.