Pex14p Peroxisomal marker | Agrisera AS08 372 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

Pex14p Peroxisomal marker

catalog :

AS08 372

quantity :

50 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

rice blast fungus, thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; rice blast fungus; 1:2000; fig 12	Zhong K, Li X, Le X, Kong X, Zhang H, Zheng X, et al. MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe oryzae. PLoS Pathog. 2016;12:e1005823 pubmed publisher
western blot; thale cress; 1:10,000; fig 4	McDonnell M, Burkhart S, Stoddard J, Wright Z, Strader L, Bartel B. The Early-Acting Peroxin PEX19 Is Redundantly Encoded, Farnesylated, and Essential for Viability in Arabidopsis thaliana. PLoS ONE. 2016;11:e0148335 pubmed publisher
western blot; thale cress; 1:10,000; fig 2c	Kao Y, Bartel B. Elevated growth temperature decreases levels of the PEX5 peroxisome-targeting signal receptor and ameliorates defects of Arabidopsis mutants with an impaired PEX4 ubiquitin-conjugating enzyme. BMC Plant Biol. 2015;15:224 pubmed publisher
western blot; thale cress; fig 7	Li F, Chung T, Pennington J, Federico M, Kaeppler H, Kaeppler S, et al. Autophagic recycling plays a central role in maize nitrogen remobilization. Plant Cell. 2015;27:1389-408 pubmed publisher
	Ling Q, Broad W, Trösch R, Töpel M, Demiral Sert T, Lymperopoulos P, et al. Ubiquitin-dependent chloroplast-associated protein degradation in plants. Science. 2019;363: pubmed publisher
	Burkhart S, Lingard M, Bartel B. Genetic dissection of peroxisome-associated matrix protein degradation in Arabidopsis thaliana. Genetics. 2013;193:125-41 pubmed publisher

product information

Product number :

AS08 372

Product name :

Pex14p Peroxisomal marker

Immunogen :

Recombinant, soluble N-terminal domain of Arabidopsis thaliana Pex14p (Q9FE40, At5g62810) that mediates PEX5 and PEX19 binding. The transmembrane and coiled-coil domains of PEX14 were replaced with a dual StrepII-His6 tag.

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4

Format :

Lyophilized

Quantity :

50 µg

Tested applications :

Western blot (WB)

Related products :

AS10 711 DEG15 endopeptidase, peroxisomal marker - with wider species reactivity than Arabidopsis thalianaAS11 1797 HPR hydroxypyruvate reductase (peroxysomal matrix marker)Plant protein extraction bufferSecondary antibodies

Recommended dilutions :

1 : 10 000 (WB)

Expected apparent MW :

55.5 75-65 kDa (depending on the gel system)

Confirmed reactivity :

Arabidopsis thaliana, Solanum tuberosum

Predicted reactivity :

Cucumis melo, Magnaporthe oryzae

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

Antibodies will detect Pex14 protein in both light- and dark-grown seedlings grown on petri dishes and in rosette leaves of adult plants grown in soil.

Selected references :

Gonzalez et al. (2018). A pex1 missense mutation improves peroxisome function in a subset of Arabidopsis pex6 mutants without restoring PEX5 recycling. Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):E3163-E3172. doi: 10.1073/pnas.1721279115. Vincent et al. (2017). A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial association. Plant J. 2017 Dec;92(6):1132-1142. doi: 10.1111/tpj.13749. Zhong et al. (2016). MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe oryzae. PLoS Pathog. 2016 Aug 24;12(8):e1005823. doi: 10.1371/journal.ppat.1005823. eCollection 2016.Li et al. (2015). A Chaperone Function of NO CATALASE ACTIVITY1 Is Required to Maintain Catalase Activity and for Multiple Stress Responses in Arabidopsis. Plant Cell. 2015 Feb 19. pii: tpc.114.135095.

Pubmed ID's :

29555730, 29044717, 27556292, 25700484

Background :

Pex14p has a protein transporter activity and is involved in protein targeting into peroxisome. Submitted protein name: Genomic DNA, chromosome 5, P1 clone:MQB2

Reconstitution :

For reconstitution add 25 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

Pex14p is prone to degradation.

UniProt number :

Q9FE40

TAIR number :

AT5G62810

more info or order :

Agrisera product webpage