antibody

HSP70 Heat shock protein 70 (cytoplasmic)

AS08 371

50 µl

polyclonal

rabbit

nonconjugated

western blot, immunoprecipitation

HSP70 Heat shock protein 70 (cytoplasmic)

KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 UniProt: F4KCE5, HSC70-2 UniProt: A0A178UTH3 and HSC70-3 Uniprot: O65719 as well as heat shock inducible Hsp70 of Arabidopsis thaliana TAIR: AT3g12580/T2E22_110 and At1g16030 and AT3g12580/T2E22_110

Rabbit

Polyclonal

Serum

Lyophilized

50 µl

Immunoprecipitation (IP), Western blot (WB)

AS08 348 anti-chloroplastic HSP70, rabbit antibodyAS08 347 anti-mitochondrial HSP70, rabbit antibodyPlant and algal protein extraction bufferSecondary antibodies

1 : 3000 - 1: 10 000, 5 µg protein/well (WB), 2-3 µl/protein extract of concentration 3-5 mg/ml

70 kDa

Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlamydomonas sp. UWO241, Cucumis sativus, E. teft, Hordeum vulgare, Medicago sativa, Salicornia sp., Silene vulgaris, Solanum lycopersicum, Pinus strobus, Polyscias elegans, Zea mays, algae: Desmodesmus subspicatus, Gracilaria vermiculophylla, phycobiont: Trebouxia TR1 and TR9, Plasmodium falciparum, Vicia faba

Ageratina adenophora, Allium sativum, Arabis alpina, Arachis diogoi, Arundo donax, Brassica napus, brassica rapa subsp. pekinensis, Camellia sinensis, Citrus sinensis, Coffea arabica, Eriobotrya japonica, Gossypium arboretum, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Lotus japonicus, Medicago sativa, medicago truncatula, Musa acuminata subsp. malaccensis, Nicotiana tabacum, Nicotiana bethamiana, Oryza sativa, Phaseolus vulgaris, Physcomitrella patens, Pinus taeda, Pisum sativum, Populus balsamifera, Populus trichocarpa, Salix gilgiana, Saussurea medusa,Solanum tuberosum, Solanum commersonii, Spinacia oleracea, Tragopogon dubius, Tragopogon porrifolius, Triticum aestivum, Vitis vinifera, Volvox sp.

No confirmed exceptions from predicted reactivity are currently known.

This product can be sold containing ProClin if requested. This antibody can be used as a marker of cytoplasmic fraction in tomato (Anfoka et al. 2015).Applied primary antibody dilution in western blot depends upon sensitivity of detection reagents (pico or femtogram for chemiluminescent detection).Immunoprecipitation protocol using Agrisera anti-Hsp70 cytosolic antibodies, see tab: protocols.

Lentini et al. (2018). Early responses to cadmium exposure in barley plants: effects on biometric and physiological parameters. Acta Physiol Plant (2018) 40: 178. https://doi.org/10.1007/s11738-018-2752-2. Balážová et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013. Yoon et al. (2018). The subfamily II catalytic subunits of protein phosphatase 2A (PP2A) are involved in cortical microtubule organization. Planta. 2018 Sep 6. doi: 10.1007/s00425-018-3000-0. Alamri et al. (2018). Nitric oxide-mediated cross-talk of proline and heat shock proteins induce thermotolerance in Vicia faba L. Environmental and Experimental Botany Available online 23 June 2018. Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117. Gorovits et al. (2017). The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation. Cell Stress Chaperones. 2017 Mar 21. doi: 10.1007/s12192-017-0766-0. Fernández-Bautista N. et al. (2017). AtHOP3, a member of the HOP family in Arabidopsis, interacts with BiP and plays a major role in the ER stress response. Plant Cell Environ. 2017 Feb 2. doi: 10.1111/pce.12927.Hammann et al. (2016). Selection of heat‑shock resistance traits during the invasion of the seaweed Gracilaria vermiculophylla. Marine Biology 163: 104.Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]Gorovits et al. (2016). Tomato yellow leaf curl virus confronts host degradation by sheltering in small/midsized protein aggregates. Virus Res. 2016 Feb 2;213:304-13. doi: 10.1016/j.virusres.2015.11.020. Epub 2015 Dec 1.Ghandi et al. (2016). Tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperature. Sci Rep. 2016 Jan 21;6:19715. doi: 10.1038/srep19715.

29957573, 30191298, 29269486, 28324352, 28155228, 27541077, 26654789, 26792235

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Q9LHA8

AT3G12580