This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
ATPase ATP synthase, whole enzyme
catalog :
AS08 370
quantity :
100 µl
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot
product information
Product number :
AS08 370
Product name :
ATPase ATP synthase, whole enzyme
Immunogen :
Purified native protein from spinach
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
100 µl
Tested applications :
Western blot (WB)
Related products :
AS08 304 anti-ATP synthase subunit alpha antibodyAS03 030 anti-ATP synthase subunit beta hen antibodyAS08 312 anti-ATP synthase subunit gamma antibodyAS05 071 anti-ATP synthase subunit c antibodyAS12 1853 anti-ATP synthase subunit c antibodyPlant and algal protein extraction bufferSecondary antibodies
Recommended dilutions :
1 : 10 000 (WB)
Expected apparent MW :
38-58 kDa
Confirmed reactivity :
Subunits alpha, beta, gamma, epsilon of Arabidopsis thaliana, Chlamydomonas reinhardtii, Spinacia oleracea, Pisum sativum, perennial grasses: Eremochloa ophiuriudes, Paspalum vaginatum
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
The prominent reaction is to a doublet around 55-58 kDa which is alpha and beta and weaker reaction to gamma subunit which is around 38 kDa.
Selected references :
Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446. Liu et al. (2011). Identification of differentially expressed salt-responsive proteins in roots of two perennial grass species contrasting in salinity tolerance. J Plant Physiol. Nov 7
Pubmed ID's :
29437989, 22070977
Background :
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.
Reconstitution :
For reconstitution add 50 µl of sterile water. To get the best possible dissolution please keep it at +4°C ON.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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