APX L-ascorbate peroxidase | Agrisera AS08 368 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

APX L-ascorbate peroxidase

catalog :

AS08 368

quantity :

50 µl

clonality :

polyclonal

host :

rabbit

conjugate :

HRP

reactivity :

thale cress, bread wheat

application :

western blot, chromatin immunoprecipitation

more info or order :

Agrisera product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; scFv; 1:2000; fig 2	Vuleta A, ManitaÅ¡eviÄ‡ JovanoviÄ‡ S, TuciÄ‡ B. Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016;100:166-173 pubmed publisher
western blot; thale cress; 1:2000; fig 4	Ostaszewska Bugajska M, Rychter A, Juszczuk I. Antioxidative and proteolytic systems protect mitochondria from oxidative damage in S-deficient Arabidopsis thaliana. J Plant Physiol. 2015;186-187:25-38 pubmed publisher
chromatin immunoprecipitation; bread wheat; fig 4	Gou J, Li K, Wu K, Wang X, Lin H, Cantu D, et al. Wheat Stripe Rust Resistance Protein WKS1 Reduces the Ability of the Thylakoid-Associated Ascorbate Peroxidase to Detoxify Reactive Oxygen Species. Plant Cell. 2015;27:1755-70 pubmed publisher
	Hu W, Wang C, Liu T, Chalifour A, Chen J, Shen Z, et al. Proteomic analysis reveals differences in tolerance to acid rain in two broad-leaf tree species, Liquidambar formosana and Schima superba. PLoS ONE. 2014;9:e102532 pubmed publisher
	An F, Fan J, Li J, Li Q, Li K, Zhu W, et al. Comparison of leaf proteomes of cassava (Manihot esculenta Crantz) cultivar NZ199 diploid and autotetraploid genotypes. PLoS ONE. 2014;9:e85991 pubmed publisher
	Chen J, Wang W, Liu T, Wu F, Zheng H. Photosynthetic and antioxidant responses of Liquidambar formosana and Schima superba seedlings to sulfuric-rich and nitric-rich simulated acid rain. Plant Physiol Biochem. 2013;64:41-51 pubmed publisher
	Juszczak I, Rudnik R, Pietzenuk B, Baier M. Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol Plant. 2012;146:53-70 pubmed publisher
	Zhang H, Tan Z, Hu L, Wang S, Luo J, Jones R. Hydrogen sulfide alleviates aluminum toxicity in germinating wheat seedlings. J Integr Plant Biol. 2010;52:556-67 pubmed publisher

product information

Product number :

AS08 368

Product name :

APX L-ascorbate peroxidase

Immunogen :

Synthetic peptide derived from Arabidopsis thaliana tAPX (acession At1g77490) and sAPX (At4g08390) protein sequences, coupled to BSA. Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cPX2 (At3g09640) and pAPX (At4g35000)

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

50 µl

Tested applications :

Western blot (WB)

Related products :

AS06 180 anti-cAPX

Recommended dilutions :

1 : 2000 (WB)

Expected apparent MW :

25-38 kDa for A. thaliana

Confirmed reactivity :

Arabidopsis thaliana, Armeria maritima, Capsicum annuum, Citrus sp., Digitaria sanguinalis, Echinochla crus-galli, Iris pumila, Liquidambar formosana, Manihot esculenta, Medicago sativa, Nicotiana tabacum thylakoid-bound APX, stromal APX; Oryza sativa, Panicum milaceum, Schima superba, Silene vulgaris, Spinacia oleracea (stromal APX, thylakoid-bound)

Predicted reactivity :

Brassica rapa subsp. oleifera Stromal APX; Glycine max, Glycine soja L-ascorbate peroxidase T, chloroplastic; Medicago truncatula thylakoid-bound APX; Pisum sativum Chloroplast stromal ascorbate peroxidase 12; Solanum lycopersicum thylakoid-bound APX; Spinacia oleracea stromal APX; Theobroma cacao L-APX T isoform 3; Vitis vinifera

Not reactive in :

Algae

Additional information :

This product can be sold containing proclin if requested

Selected references :

BalfagÃ³n et al. (2018). Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. 2018 Cunha et al. (2016). Salinity and osmotic stress trigger different antioxidant responses related to cytosolic ascorbate peroxidase knockdown in rice roots. Environmental and Experimental Botany, Volume 131, November 2016, Pages 58-67. Ko et al. (2016). Constitutive expression of a fungus-inducible carboxylesterase improves disease resistance in transgenic pepper plants. Planta. 2016 Aug; 244(2):379-92. doi: 10.1007/s00425-016-2514-6. Epub 2016 Apr 13.Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1â€“12.Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29.Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.

Pubmed ID's :

29609175, 27074836, 27124767, 26841194, 25070267, 24727655

Background :

APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX).

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt number :

Q42593 , Q42592 , Q05431 , Q1PER6 , Q42564

TAIR number :

AT1G77490

more info or order :

Agrisera product webpage