GTA MCP Gene Transfer Agent (GTA) major capsid protein (MCP)

product summary

company name :

Agrisera

product type :

antibody

product name :

GTA MCP Gene Transfer Agent (GTA) major capsid protein (MCP)

catalog :

AS08 365

quantity :

200 µl

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

Rhodobacter sphaeroides 2.4.1

application :

western blot

more info or order :

Agrisera product webpage

citations: 3

Reference
Westbye A, Leung M, Florizone S, Taylor T, Johnson J, Fogg P, et al. Phosphate concentration and the putative sensor kinase protein CckA modulate cell lysis and release of the Rhodobacter capsulatus gene transfer agent. J Bacteriol. 2013;195:5025-40 pubmed publisher
Hynes A, Mercer R, Watton D, Buckley C, Lang A. DNA packaging bias and differential expression of gene transfer agent genes within a population during production and release of the Rhodobacter capsulatus gene transfer agent, RcGTA. Mol Microbiol. 2012;85:314-25 pubmed publisher
Petersen J, Brinkmann H, Pradella S. Diversity and evolution of repABC type plasmids in Rhodobacterales. Environ Microbiol. 2009;11:2627-38 pubmed publisher

product information

Product number :

AS08 365

Product name :

GTA MCP Gene Transfer Agent (GTA) major capsid protein (MCP)

Immunogen :

KLH-conjugated conserved peptide sequence found in the Gene Transfer Agent (GTA) major capsid protein (MCP) encoded in Bacteria within the Rhodobacterales order of the class alpha-Proteobacteria including Rhodobacter sphaeroidesQ3J3K4

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

200 µl

Tested applications :

Western blot (WB)

Recommended dilutions :

1 : 1000 (WB)

Expected apparent MW :

31.4 32 kDa (predicted mature capsid protein of Rhodobacter capsulatus)

Confirmed reactivity :

Rhodobacter capsulatus, Ruegeria pomeroyi DSS-3

Predicted reactivity :

Rhodobacterales

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Selected references :

Tomasch et al. (2018). Packaging of Dinoroseobacter shibae DNA into Gene Transfer Agent Particles Is Not Random. Genome Biol Evol. 2018 Jan 1;10(1):359-369. doi: 10.1093/gbe/evy005. Mercer and Lang (2014). Identification of a predicted partner-switching system that affects production of the gene transfer agent RcGTA and stationary phase viability in Rhodobacter capsulatus. BMC Microbiol. 2014 Mar 19;14(1):71.

Pubmed ID's :

29325123, 24645667

Background :

Gene Transfer Agents (GTAs) are bacteriophage-like particles which function as a means to package and transfer genomic DNA between bacterial cells.GTA-mediated gene transfer might be an important phenomenon in natural microbial communities.

Reconstitution :

For reconstitution add 200 µl of sterile distilled water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt number :

Q3J3K4

more info or order :

Agrisera product webpage