product summary
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company name :
Agrisera
product type :
antibody
product name :
Toc75 Protein TOC75-3, chloroplastic, POTRA domain 3
catalog :
AS08 351
quantity :
50 µl
price :
302 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, flow cytometry
more info or order :
citations: 2
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product information
Product number :
AS08 351
Product name :
Toc75 Protein TOC75-3, chloroplastic, POTRA domain 3
Quantity :
50 µl
List Price :
302 USD
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
Immunogen :
Pisum sativum TOC75; Predicted POTRA Domain #3; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. UniProt: Q43715
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Tested applications :
Flow Cytometry (Flow cyt), Immunolocalization (IL), Western blot (WB)
Recommended dilutions :
1 : 500 (Flow cyt), 1 : 100 (IL), 1 : 2000-1 : 100 000 (WB)
Expected apparent MW :
88 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
Confirmed reactivity :
Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
Predicted reactivity :
Aegilops tauschii, Ananas comosus, Anthurium amnicola, Capsicum annuum, Catalpa bungei, Cicer arietinum, Cucumis melo, Glycine soja, Gossypium arboreum, Medicago truncatula, Morus notabilis, Nelumbo nucifera, Nicotiana sylvestris, Noccaea caerulescens, Populus trichocarpa, Ricinus communis, Sorghum bicolor, Theobroma cacao, Zostera marina, Vigna radiata, Vitis vinifera
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction
Related products :
Plant protein extraction bufferSecondary antibodies
Selected references :
Sasaki and Yamamoto (2015). Arabidopsis LAZY1 is a peripheral membrane protein of which the carboxy-terminal fragment potentially interacts with microtubules. Copyright © 2015 The Japanese Society for Plant Cell and Molecular Biology, Plant Biotechnology, 32, 1-6 (2015) DOI: 10.5511/plantbiotechnology.15.0116aVera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.Hsueh et al. (2014). The chloroplast outer envelope protein P39 in Arabidopsis thaliana belongs to the Omp85 protein family. Proteins. 2014 Nov 17. doi: 10.1002/prot.24725.
Background :
OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303); FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
UniProt number :
Q43715
TAIR number :
AT3G46740
Commodity Code :
38220000
Our Category :
Chaperones/Transporters
Research area :
Plant Biology, Photosynthesis
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.