product summary
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company name :
Agrisera
product type :
antibody
product name :
Toc75 Protein TOC75-3, chloroplastic, POTRA domain 1
catalog :
AS08 345
quantity :
200 µl
price :
302 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, flow cytometry
more info or order :
product information
Product number :
AS08 345
Product name :
Toc75 Protein TOC75-3, chloroplastic, POTRA domain 1
Quantity :
200 µl
List Price :
302 USD
Immunogen :
psTOC75; Predicted POTRA Domain #1; Amino acids, 158-241; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Tested applications :
Flow cytometry (Flow cyt), Immunolocalization (IL),Western blot (WB)
Recommended dilutions :
1 : 500 (Flow cyt), 1 : 100 (IL), 1 : 2000-1 : 100 000 (WB)
Expected apparent MW :
88 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
Confirmed reactivity :
Pisum sativum, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus
Predicted reactivity :
Ricinus communis, Vitis vinifera, Populus trichocarpa
Not reactive in :
Oryza sativa, Physcomitrella patens
Additional information :
Antibody detects Toc75 POTRA domain 1 as purified protein, in chloroplast fraction and in crude envelope fraction
Related products :
Plant protein extraction bufferSecondary antibodies
Background :
OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303); FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
Reconstitution :
For reconstitution add 200 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
UniProt number :
158241 , Q43715
Commodity Code :
38220000
Our Category :
Chaperones/Transporters
Research area :
Plant Biology, Photosynthesis
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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