product summary
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company name :
Agrisera
product type :
antibody
product name :
VRN2 Vernalization protein 2
catalog :
AS08 341
quantity :
50 µg
price :
349 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
application :
western blot, ELISA
more info or order :
product information
Product number :
AS08 341
Product name :
VRN2 Vernalization protein 2
Quantity :
50 µg
List Price :
349 USD
Immunogen :
KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence
Host :
Mouse
Clonality :
Monoclonal
Purity :
Total IgG. Protein G purified purified from Cell culture supernatant.
Format :
Lyophilized
Tested applications :
ELISA (ELISA), Western blot (WB)
Recommended dilutions :
1 : 10 000 (ELISA), 1 : 1000 (WB)
Expected apparent MW :
23,7 kDa
Confirmed reactivity :
Triticum aestivum
Predicted reactivity :
Triticum monococcum
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
There is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T, monococcum
Background :
Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.
Commodity Code :
38220000
Our Category :
Photomorphogenesis
Research area :
Plant Biology
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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