NAB1 nucleic acid binding protein 1, Chlamydomonas | Agrisera AS08 333 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Agrisera

product type :

antibody

product name :

NAB1 nucleic acid binding protein 1, Chlamydomonas

catalog :

AS08 333

quantity :

50 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

Chlamydomonas reinhardtii

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 3

Reference
Liu P, Lou X, Wingfield J, Lin J, Nicastro D, Lechtreck K. Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia. J Cell Biol. 2020;219: pubmed publisher
Perlaza K, Toutkoushian H, Boone M, Lam M, Iwai M, Jonikas M, et al. The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. elife. 2019;8: pubmed publisher
Findinier J, Delevoye C, Cohen M. The dynamin-like protein Fzl promotes thylakoid fusion and resistance to light stress in Chlamydomonas reinhardtii. PLoS Genet. 2019;15:e1008047 pubmed publisher

product information

Product number :

AS08 333

Product name :

NAB1 nucleic acid binding protein 1, Chlamydomonas

Immunogen :

His-tagged recombinant NAB1 from Chlamydomonas reinhardtii UniProt:Q8GV23

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

50 µl

Tested applications :

Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Related products :

Collection of antibodies to Chlamydomonas proteins

Recommended dilutions :

1: 500 (IF), 1 : 10 000 (WB)

Expected apparent MW :

26.5 kDa 26 kDa (for Chlamydomonas reinhardtii)

Confirmed reactivity :

Chlamydomonas reinhardtii

Predicted reactivity :

Chlamydomonas reinhardtii

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known

Selected references :

Lechtreck et al. (2022) Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for intraflagellar transport of radial spokes. Elife. 2022 Jan 4;11:e74993. doi: 10.7554/eLife.74993. PMID: 34982025; PMCID: PMC8789290.Perlaza(2021). Organelle Size and Quality Control in Chlamydomonas Reinhardtii. UCSF. ProQuest ID: Perlaza_ucsf_0034D_12217. Merritt ID: ark:/13030/m5257z1d. Retrieved from https://escholarship.org/uc/item/1jg3874hLiu et al. (2020). Chlamydomonas PKD2 Organizes Mastigonemes, Hair-Like Glycoprotein Polymers on Cilia. J Cell Biol . 2020 Jun 1;219(6):e202001122. doi: 10.1083/jcb.202001122Perlaza et al. (2019). The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577. (immunofluorescence)Findinier et al. (2019). The dynamin-like protein Fzl promotes thylakoid fusion and resistance to light stress in Chlamydomonas reinhardtii. PLoS Genet. 2019 Mar 15;15(3):e1008047. doi: 10.1371/journal.pgen.1008047.

Background :

In Chlamydomonas reinhardtii NAB1 (nucleic acid binding protein 1) interacts in the cytosol with mRNAs coding for light-harvesting proteins of Photosystem II. NAB1 has structural similarities (cold-shock domain, CSD) to the FRGY2-protein from Xenopus laevis. According to immunolocalization studies NAB1 protein was exclusively found in cytosol, Mussgnug et al. (2005).

Reconstitution :

For reconstitution add 50 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

NAB1 can be used as a marker of cytoplasm in Chlamydomonas reinhardii.

UniProt number :

Q8GV23

Research area :

Chlamydomonas reinhardtii