product summary
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company name :
Agrisera
product type :
antibody
product name :
L7/L12 Ribosomal protein
catalog :
AS08 331
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot
more info or order :
product information
Product number :
AS08 331
Product name :
L7/L12 Ribosomal protein
Immunogen :
Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
Western blot (WB)
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Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)
Confirmed reactivity :
Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
Background :
Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Name of this antibody has been changed from L-30 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul;29(1):68-88.
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.