Abeta (1-42) Amyloid-beta peptide 1-42 | Agrisera AS08 328 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

Abeta (1-42) Amyloid-beta peptide 1-42

catalog :

AS08 328

quantity :

100 µl

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry - paraffin section, dot blot

more info or order :

Agrisera product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:200	Wang C, Klechikov A, Gharibyan A, Wärmländer S, Jarvet J, Zhao L, et al. The role of pro-inflammatory S100A9 in Alzheimer's disease amyloid-neuroinflammatory cascade. Acta Neuropathol. 2014;127:507-22 pubmed publisher
	Lindhagen Persson M, Brannstrom K, Vestling M, Steinitz M, Olofsson A. Amyloid-? oligomer specificity mediated by the IgM isotype--implications for a specific protective mechanism exerted by endogenous auto-antibodies. PLoS ONE. 2010;5:e13928 pubmed publisher

product information

Product number :

AS08 328

Product name :

Abeta (1-42) Amyloid-beta peptide 1-42

Immunogen :

synthetic peptide chosen from human Abeta (1-42) protein. Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

100 µl

Tested applications :

Dot Blot (Dot), ELISA (ELISA), Immunolocalization (IL), Western blot (WB)

Related products :

Alzheimer available antibodies for Alzheimer's disease

Recommended dilutions :

1 : 1000 (DB), 1 : 3000 (ELISA), 1-2 µg/ml (IL)

Expected apparent MW :

4.5 kDa

Confirmed reactivity :

Human

Predicted reactivity :

Bovine, Chicken, Dog, Porcine, Rabbit

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

The antibody can detect Abeta (1-42), Abeta (1-28) Abeta (1-20) and Abeta (1-17). This product exhibits a low reactivity to monomeric Abeta (1-42) as determined by SDS-PAGE and Western blotting.Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.

Selected references :

Lindhagen-Persson et al. (2010). Amyloid-beta oligomer specificity mediated by the IgM isotype--implications for a specific protective mechanism exerted by endogenous auto-antibodies. PLoS One. 2010 Nov 10;5(11):e13928. doi: 10.1371/journal.pone.0013928.

Pubmed ID's :

21085663

Background :

Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein P05067

Reconstitution :

For reconstitution add 100 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

more info or order :

Agrisera product webpage