product summary
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company name :
Agrisera
product type :
antibody
product name :
Sar1 Secretion-associated and Ras-related protein 1
catalog :
AS08 326
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
citations: 2
| Reference |
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product information
Product number :
AS08 326
Product name :
Sar1 Secretion-associated and Ras-related protein 1
Immunogen :
GST fusion of full length recombinant Sar1 of Arabidopsis thaliana Q8VYP7, At3g62560
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
Western blot (WB), Immunogold (IG)
Related products :
AS08 327 anti-Sec21p gamma subunit, COP vesicles, Golgi marker, rabbit antibodyAS08 325 anti-Arf1 ADP-ribosylation factor 1 rabbit antibodySecondary antibodies
Recommended dilutions :
1 : 50 (IG), 1 : 500 (WB)
Expected apparent MW :
21 kDa (Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis, Pteris vittata (fern), Panicum miliaceum, Panicum maximum, Petunia hybrida, Triticum aestivum, Zea mays
Predicted reactivity :
Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa, Capsella rubella, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Populus trichocarpa, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera
Not reactive in :
Nicotiana tabacum
Additional information :
Immunolocalization method with Sar1 antibodies is described in: Yao-dong Yang (2005). Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites. Plant Cell. 2005 May;17(5):1513-31. Epub 2005 Apr 1.
Selected references :
Liu et al. (2014). SCFSLF-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida. Front. Genet., 22 July 2014 doi: 10.3389/fgene.2014.00228 Shen et al. (2014). The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L. J Proteomics. 2014 Feb 4. pii: S1874-3919(14)00047-5. doi: 10.1016/j.jprot.2014.01.029.
Pubmed ID's :
24508335
Background :
Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins – Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES. LR White resin has been used. Tested species were: Triticum aestivum, Panicum miliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis. Publication in preparation.
UniProt number :
Q8VYP7
TAIR number :
AT3G62560
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.