ARF1 ADP-ribosylation factor 1 | Agrisera AS08 325 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

ARF1 ADP-ribosylation factor 1

catalog :

AS08 325

quantity :

50 µl

price :

302 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, immunocytochemistry

more info or order :

Agrisera product webpage

citations: 7

Reference
Hurny A, Cuesta C, Cavallari N, Otvös K, Duclercq J, Dokládal L, et al. SYNERGISTIC ON AUXIN AND CYTOKININ 1 positively regulates growth and attenuates soil pathogen resistance. Nat Commun. 2020;11:2170 pubmed publisher
Kuang L, Chen S, Guo Y, Ma H. Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019;10:641 pubmed publisher
Singh M, Richter S, Beckmann H, Kientz M, Stierhof Y, Anders N, et al. A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018;14:e1007795 pubmed publisher
Deppe J, Rabbat R, Hortensteiner S, Keller B, Martinoia E, Lopez Marques R. The wheat ABC transporter Lr34 modifies the lipid environment at the plasma membrane. J Biol Chem. 2018;293:18667-18679 pubmed publisher
Vincent T, Vingadassalon A, Ubrig E, Azeredo K, Srour O, Cognat V, et al. A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial association. Plant J. 2017;: pubmed publisher
Lynch J, Orlova I, Zhao C, Guo L, Jaini R, Maeda H, et al. Multifaceted plant responses to circumvent Phe hyperaccumulation by downregulation of flux through the shikimate pathway and by vacuolar Phe sequestration. Plant J. 2017;92:939-950 pubmed publisher
Ma J, Cheng Z, Chen J, Shen J, Zhang B, Ren Y, et al. Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of Exocytosis. PLoS ONE. 2016;11:e0153119 pubmed publisher

product information

Product number :

AS08 325

Product name :

ARF1 ADP-ribosylation factor 1

Quantity :

50 µl

List Price :

302 USD

Special price 2+ units (List Price) :

226

Special price 3+ units (List Price) :

205

Immunogen :

Recombinant GST fusion of full length of Arabidopsis thaliana ARF1 (P36397, AT2G47170)

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Immunofluorescence (IF), Immunogold (IG), Western blot (WB)

Recommended dilutions :

1 : 1000 (IF), 1 : 100 (IG), 1 : 1000 (WB)

Expected apparent MW :

21 kDa (Arabidopsis thaliana)

Confirmed reactivity :

Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Elaeis sp. , Lilium longiflorum, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Petunia hybrida cv Mitchell, Physcomitrium patens, Solanum tuberosum

Predicted reactivity :

Brassica juncea, Brassica napus, Capsella rubella, Capsicum annum, Cucumis sp., Daucus carota, Elaeis guineensis, Glycine max, Helleborus orientalis, Hordeum vulgare, Medicago truncatula, Nannochloropsis gaditana, Nicotiana benthamina, Ostreococcus tauri, Populus trichocarpa, Triticum aestivum, Zea mays

Not reactive in :

Microsporidia sp.

Additional information :

References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36.Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.

Related products :

Plant and algal protein extraction buffer Secondary antibodies

Selected references :

Farago et al. (2022) Small paraquat resistance proteins modulate paraquat and ABA responses and confer drought tolerance to overexpressing Arabidopsis plants. Plant Cell Environ. 2022 Jul;45(7):1985-2003. doi: 10.1111/pce.14338. Epub 2022 Apr 29. PMID: 35486392; PMCID: PMC9324991.Narasimhan et al. (2021) Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. Plant Physiol. 2021 Mar 18:kiab134. doi: 10.1093/plphys/kiab134. Epub ahead of print. PMID: 33734402.Hurny et al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization)Kuang et al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.Singh et al. (2018). A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795.

Background :

The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.

Reconstitution :

For reconstitution add 50 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

Cellular [compartment marker] of Golgi in immunolocalization and COP1 in western blot

UniProt number :

P36397

TAIR number :

AT2G47170

Commodity Code :

38220000

Our Category :

Endomembrane system

Research area :

Cell Biology

more info or order :

Agrisera product webpage